MtnW

• Description: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein
  
  

Gene name	mtnW
Synonyms	ykrW
Essential	no
Product	2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Function	methionine salvage
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	44 kDa, 5.284
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	mtnE, mtnX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13590

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-(methylthio)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (according to Swiss-Prot)

• Protein family: Type IV subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2ZVI PubMed

• UniProt: O31666

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: