MalK

• Description: two-component sensor kinase, regulation of malate uptake
  
  

Gene name	malK
Synonyms	yufL
Essential	no
Product	two-component sensor kinase
Function	regulation of malate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	58 kDa, 6.947
Gene length, protein length	1599 bp, 533 aa
Immediate neighbours	yufK, malR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU31520

Phenotypes of a mutant

unable to use malate as the single carbon source PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

utilization of specific carbon sources, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of MalR

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MalK-MalR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05250

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France