DapB

• Description: dihydrodipicolinate reductase (NADPH)
  
  

Gene name	dapB
Synonyms
Essential	yes PubMed
Product	dihydrodipicolinate reductase (NADPH)
Function	biosynthesis of lysine and peptidoglycan
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	29 kDa, 5.057
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	mgsA, ypjD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22490

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2,3,4,5-tetrahydrodipicolinate + NAD(P)⁺ = 2,3-dihydrodipicolinate + NAD(P)H (according to Swiss-Prot)

• Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YL6 (from Mycobacterium tuberculosis, 46% identity, 67% similarity) PubMed

• UniProt: P42976

• KEGG entry: [2]

• E.C. number: 1.3.1.26

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Operon and expression

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)