AroK

• Description: shikimate kinase
  
  

Gene name	aroK
Synonyms	aroI
Essential	no
Product	shikimate kinase
Function	biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	21 kDa, 5.969
Gene length, protein length	558 bp, 186 aa
Immediate neighbours	tmrB, ycgJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + shikimate = ADP + shikimate 3-phosphate (according to Swiss-Prot)

• Protein family: shikimate kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37944

• KEGG entry: [3]

• E.C. number: 2.7.1.71

Expression and regulation

• Operon: aroK (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

L Huang, A L Montoya, E W Nester
Purification and characterization of shikimate kinase enzyme activity in Bacillus subtilis.
J Biol Chem: 1975, 250(19);7675-81
[PubMed:170268] [WorldCat.org] (P p)

J A Hoch, E W Nester
Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1973, 116(1);59-66
[PubMed:4200844] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen, D S Nasser
Regulation of enzyme synthesis in the aromatic amino acid pathway of Bacillus subtilus.
J Bacteriol: 1969, 97(1);83-90
[PubMed:4974400] [WorldCat.org] [DOI] (P p)