Difference between revisions of "LipA"
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| − | + | * '''Description:''' [[trigger enzymes|trigger enzyme]]: lipoic acid synthase and regulator of ''[[comEA|comE]]'' operon expression <br/><br/> | |
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| − | * '''Description:''' [[trigger enzymes|trigger enzyme]]: lipoic acid synthase and regulator of ''[[comEA|comE]]'' operon expression | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yutC]]'', ''[[lytH]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yutC]]'', ''[[lytH]]'' | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+& | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15223]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
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| − | |colspan="2" | '''Genetic context''' | + | |colspan="2" | '''Genetic context''' <br/> [[Image:lipA_context.gif]] |
| − | + | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | |
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=The gene= | =The gene= | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H | + | * '''Catalyzed reaction/ biological activity:''' Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for ''[[comEA]]'' transcription [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed] |
* '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot) | * '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot) | ||
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=References= | =References= | ||
| − | + | <pubmed>18957862 19028902, 19820084 </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:37, 24 November 2010
- Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression
| Gene name | lipA |
| Synonyms | yutB |
| Essential | Yes PubMed |
| Product | trigger enzyme: lipoic acid synthase |
| Function | lipid metabolism, required for the synthesis of branched-chain amino acids |
| MW, pI | 33 kDa, 8.251 |
| Gene length, protein length | 894 bp, 298 aa |
| Immediate neighbours | yutC, lytH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU32330
Phenotypes of a mutant
reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed
- Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure:
- E.C. number: 2.8.1.8 3.1.1.3]
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Natalia Martin, Esteban Lombardía, Silvia G Altabe, Diego de Mendoza, María C Mansilla
A lipA (yutB) mutant, encoding lipoic acid synthase, provides insight into the interplay between branched-chain and unsaturated fatty acid biosynthesis in Bacillus subtilis.
J Bacteriol: 2009, 191(24);7447-55
[PubMed:19820084]
[WorldCat.org]
[DOI]
(I p)
Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902]
[WorldCat.org]
[DOI]
(I p)
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
