Difference between revisions of "ClpP"
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[ClpE]]-[[ClpP]], [[ClpC]]-[[ClpP]], [[ClpX]]-[[ClpP]] | + | * '''Interactions:''' |
| + | ** [[ClpE]]-[[ClpP]], [[ClpC]]-[[ClpP]], [[ClpX]]-[[ClpP]] | ||
| + | ** [[CtsR]]-[[ClpP]]/[[ClpC]] (degradation of [[CtsR]]) {{PubMed|11179229,16163393,17380125}} | ||
| + | ** [[McsB]]-[[ClpP]]/[[ClpC]] (rapid degradation of non-phosphorylated McsB) {{PubMed|20852588}} | ||
* '''Localization:''' cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [[ClpX]], [[ClpC]] and [[ClpE]] {{PubMed|18786145,18689473}} | * '''Localization:''' cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [[ClpX]], [[ClpC]] and [[ClpE]] {{PubMed|18786145,18689473}} | ||
| Line 134: | Line 137: | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>17380125,9643546,10809708,11807061,9987115,14679237,18689476,15317791,17586624,11684022,12923101,17560370,12598648,16899079,19226326,9890793, 20070525,9987115,11544224 14763982 9643546, 19767395 11112444 9535081 18689473 20049702 20305655 </pubmed> | + | <pubmed>17380125,9643546,10809708,11807061,9987115,14679237,18689476,15317791,17586624,11684022,12923101,17560370,12598648,16899079,19226326,9890793, 20070525,9987115,11544224 14763982 9643546, 19767395 11112444 9535081 18689473 20049702 20305655 20852588 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:55, 26 October 2010
- Description: ATP-dependent Clp protease proteolytic subunit (class III heat-shock protein)
| Gene name | clpP |
| Synonyms | yvdN |
| Essential | no |
| Product | ATP-dependent Clp protease proteolytic subunit |
| Function | protein degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Phosphorelay, Stress | |
| MW, pI | 21 kDa, 5.008 |
| Gene length, protein length | 591 bp, 197 aa |
| Immediate neighbours | trnQ-Arg, pgcM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU34540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of proteins to small peptides in the presence of ATP and magnesium (according to Swiss-Prot) endopeptidase/proteolysis
- Protein family: peptidase S14 family (according to Swiss-Prot) ClpP (IPR001907) InterPro, (PF00574) PFAM
- Paralogous protein(s):
Targets of ClpC-ClpP-dependent protein degradation
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with ClpX, ClpC and ClpE PubMed
Database entries
- UniProt: P80244
- KEGG entry: [3]
- E.C. number: 3.4.21.92
Additional information
Expression and regulation
- Operon: clpP PubMed
- Additional information:
Biological materials
- Mutant:
- clpP::spec and clpP::cat, available in the Leendert Hamoen lab
- GP551 (spc), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion: C-terminal GFP fusions (both single copy and as 2th copy in amyE locus, also as CFP and YFP variants) available in the Leendert Hamoen lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage
Your additional remarks
References
Reviews
Noël Molière, Kürşad Turgay
Chaperone-protease systems in regulation and protein quality control in Bacillus subtilis.
Res Microbiol: 2009, 160(9);637-44
[PubMed:19781636]
[WorldCat.org]
[DOI]
(I p)
Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260]
[WorldCat.org]
[DOI]
(I p)
Original Publications
