Difference between revisions of "PcrA"
| Line 80: | Line 80: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PJR 2PJR] ( | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PJR 2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1PJR 1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'') |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34580 O34580] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O34580 O34580] | ||
| Line 126: | Line 126: | ||
<pubmed> 19943906 </pubmed> | <pubmed> 19943906 </pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>12073041,9701819 ,16267290, 19943900 20723756 </pubmed> | + | <pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:54, 15 October 2010
- Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
| Gene name | pcrA |
| Synonyms | yerF |
| Essential | yes PubMed |
| Product | ATP-dependent DNA helicase |
| Function | plasmid rolling-circle replication, conjugative transfer of ICEBs1 |
| MW, pI | 83 kDa, 5.767 |
| Gene length, protein length | 2217 bp, 739 aa |
| Immediate neighbours | pcrB, ligA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU06610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
- UniProt: O34580
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906]
[WorldCat.org]
[DOI]
(I p)
Original Publications
