AmyE

• Description: alpha-amylase
  
  

Gene name	amyE
Synonyms	amyA
Essential	no
Product	alpha-amylase)
Function	starch degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	72 kDa, 5.85
Gene length, protein length	1980 bp, 660 aa
Immediate neighbours	ycgB, ldh
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 1BAG (complex with maltopentaose)

• UniProt: P00691

• KEGG entry: [3]

• E.C. number: 3.2.1.1

Expression and regulation

• Operon: amyE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP550 (cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: