GlcT

• Description: Transcriptional antiterminator , controls expression of the ptsG-ptsH-ptsI operon
  
  

Gene name	glcT
Synonyms	ykwA
Essential	no
Product	transcriptional antiterminator of the ptsG-ptsH-ptsI operon
Function	control of glucose uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	33,0 kDa, 7.01
Gene length, protein length	855 bp, 285 amino acids
Immediate neighbours	ykvZ, ptsG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13880

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription antiterminator , RNA-binding protein, binds the ptsG RAT sequence

• Protein family: transcription antiterminator of the BglG/ SacY family

• Paralogous protein(s): LicT, SacT, SacY

Extended information on the protein

• Kinetic information:

• Domains:
  • RNA-binding domain (N-terminal, constitutive antiterminator)
  • 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)

• Modification: phosphorylation (His104)

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PtsH-GlcT

• Localization:

Database entries

• Structure: 3GWH (PRD-II) PubMed

• UniProt: O31691

• KEGG entry: [2]

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP109 (in frame deletion), GP778 (replacement of glcT and the ptsGHI operon by a spc cassette), available in Stülke lab

• Expression vector:
  • pGP124 (full length, in pWH844), available in Stülke lab
  • pGP114 (amino acids 1-60, RNA-binding domain, in pWH844), available in Stülke lab
  • pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in pWH844), available in Stülke lab
  • pGP164 (both PRDs, in pWH844), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in pWH844), available in Stülke lab
  • pGP424 (PRDI, in pWH844), available in Stülke lab
  • pGP425 (PRDII, in pWH844), available in Stülke lab
  • pGP442 (PRDI, in pGP570, with thrombin cleavage site), available in Stülke lab
  • pGP443 (PRDII, in pGP570, with thrombin cleavage site), available in Stülke lab
  • pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in pGP574), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

References

Reviews

Fabian M Commichau, Jörg Stülke
Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression.
Mol Microbiol: 2008, 67(4);692-702
[PubMed:18086213] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)