Difference between revisions of "LcfB"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' long-chain fatty-acid-CoA ligase <br/><br/>
+
* '''Description:''' long-chain fatty-acid-CoA ligase, involved in surfactin production  <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 56: Line 56:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis {{PubMed|20797616}}
  
 
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
 
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[LcfA]], [[YhfT]], [[YngI]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 106: Line 106:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 124: Line 124:
 
=References=
 
=References=
  
<pubmed>17189250,17919287 </pubmed>
+
<pubmed>17189250,17919287 20797616</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:20, 1 September 2010

  • Description: long-chain fatty-acid-CoA ligase, involved in surfactin production

Gene name lcfB
Synonyms yhfL
Essential no
Product long-chain fatty-acid-CoA ligase
Function fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 56 kDa, 5.301
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yhfK, yhfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhfL context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU10270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of long-chain fatty acids (FadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616] [WorldCat.org] [DOI] (I p)

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)