Difference between revisions of "AlkA"
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* '''Operon:''' [[alkA]] {{PubMed|8376346}} | * '''Operon:''' [[alkA]] {{PubMed|8376346}} | ||
| − | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8376346 | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8376346}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Regulatory mechanism:''' [[AdaA]]: positive regulation {{PubMed|8376346}} | * '''Regulatory mechanism:''' [[AdaA]]: positive regulation {{PubMed|8376346}} | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 19:49, 5 July 2010
- Description: DNA-3-methyladenine glycosylase
| Gene name | alkA |
| Synonyms | |
| Essential | no |
| Product | DNA-3-methyladenine glycosylase |
| Function | adaptive response to alkylative DNA damage |
| MW, pI | 35 kDa, 8.147 |
| Gene length, protein length | 909 bp, 303 aa |
| Immediate neighbours | ybbU, adaA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU01800
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)
- Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P37878
- KEGG entry: [3]
- E.C. number: 3.2.2.21
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346]
[WorldCat.org]
[DOI]
(P p)
