Difference between revisions of "List of plasmids"
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* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter | * [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter | ||
* [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter | * [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter | ||
| + | * [[pGP1751]]: ''pckA'' ''SalI'' + ''SphI'' cloned into [[pGP1331]] | ||
==Expression/ purification in ''B. subtilis''== | ==Expression/ purification in ''B. subtilis''== | ||
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*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein | *[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein | ||
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein | *[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein | ||
| + | * [[pGP1753]]: ''pckA'' ''XbaI'' + ''PstI'' cloned into [[pGP380]] | ||
==Expression/ purification in ''E. coli''== | ==Expression/ purification in ''E. coli''== | ||
Revision as of 14:57, 5 May 2010
Here you can find a list of Bacillus subtilis plasmids:
Contents
Fusion to reporter genes
- pAC5: translational lacZ-fusions (chloramphenicol resistence)
- pAC6: transcriptional lacZ-fusions (cat resistence)
- pAC7: translational lacZ-fusions (kanamycin resistence)
- pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
Labelling of a protein with a triple FLAG tag
- pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
- pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
- pGP1751: pckA SalI + SphI cloned into pGP1331
Expression/ purification in B. subtilis
- pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
- pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
- pGP1753: pckA XbaI + PstI cloned into pGP380
Expression/ purification in E. coli
- pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
- pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
- pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
- pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
- pGP1755: expression of a mutated malatedehydrogenase from B. subtilis in E. coli; mdh (Ser-149-Ala) Bam+Pst in pWH844
