Difference between revisions of "FbaA"

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* '''Mutant:'''  
 
* '''Mutant:'''  
 +
* GP591 (''fbaA''::''cat'')
 +
* GP596 (''fbaA''::''ery'')
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  

Revision as of 08:10, 19 March 2010

  • Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme

Gene name fbaA
Synonyms fba, fba1, tsr
Essential yes
Product fructose-1,6-bisphosphate aldolase
Function enzyme in glycolysis/ gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 30,2 kDa, 5.03
Gene length, protein length 855 bp, 285 amino acids
Immediate neighbours ywjH, spo0F
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FbaA context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU37120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
  • Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
  • Paralogous protein(s): FbaB

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten PubMed
  • Domains:
    • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
  • Modification: phosphorylation on Thr-212 and Thr-234 PubMed
  • Cofactor(s): Zn2+ (Metalloenzyme)
  • Effectors of protein activity:
    • Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate PubMed PubMed
    • Activated by NH4+ PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure: 2FJK (from Thermus caldophilus) PubMed
  • KEGG entry: [3]

Additional information

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • GP591 (fbaA::cat)
  • GP596 (fbaA::ery)
  • Expression vector:
    • for expression in B. subtilis, in pBQ200: pGP1423, available in Stülke lab
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Stülke lab
  • lacZ fusion: pGP601 (in pAC6)
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References