Difference between revisions of "AroE"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
m (Database entries)
Line 78: Line 78:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1RF5 1RF5] (from ''Streptococcus pneumoniae'', 52% identity, 69% similarity) {{PubMed|14763973}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P20691 P20691]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P20691 P20691]

Revision as of 11:08, 19 February 2010

  • Description: 3-phosphoshikimate 1-carboxyvinyltransferase

Gene name aroE
Synonyms
Essential no
Product 3-phosphoshikimate 1-carboxyvinyltransferase
Function biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 45 kDa, 6.341
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours ypiA, tyrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AroE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU22600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate (according to Swiss-Prot)
  • Protein family: ABC transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1RF5 (from Streptococcus pneumoniae, 52% identity, 69% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References