Difference between revisions of "TyrZ"
(→Expression and regulation) |
|||
| Line 92: | Line 92: | ||
* '''Operon:''' | * '''Operon:''' | ||
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' ''[[tyrZ]]'' {{PubMed|1735721}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** induced by tyrosine limitation ([[T-box]]) {{PubMed|19258532}} | + | ** induced by tyrosine limitation ([[T-box]]) {{PubMed|19258532,1735721}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532}} | + | ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532,1735721}} |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 122: | Line 122: | ||
=References= | =References= | ||
| − | <pubmed>19258532,, </pubmed> | + | <pubmed>19258532,1735721 , </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:09, 6 February 2010
- Description: tyrosyl-tRNA synthetase (minor)
| Gene name | tyrZ |
| Synonyms | ipa-9r, tyrS1, tyrT |
| Essential | no |
| Product | tyrosyl-tRNA synthetase (minor) |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 46 kDa, 6.661 |
| Gene length, protein length | 1239 bp, 413 aa |
| Immediate neighbours | ywaE, ywaD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU38460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
- Protein family: TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P25151
- KEGG entry: [3]
- E.C. number: 6.1.1.1 6.1.1.1]
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information:
Biological materials
- Mutant: QB53532 (aphA3), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
T M Henkin, B L Glass, F J Grundy
Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory sequence in multiple tRNA synthetase genes.
J Bacteriol: 1992, 174(4);1299-306
[PubMed:1735721]
[WorldCat.org]
[DOI]
(P p)
