Difference between revisions of "DnaC"
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| − | * '''Description:''' replicative DNA helicase <br/><br/> | + | * '''Description:''' replicative DNA helicase, part of the [[replisome]] <br/><br/> |
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[DnaB]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11250911 PubMed], [[DnaI]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed] | + | * '''Interactions:''' [[DnaB]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11250911 PubMed], [[DnaI]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed], part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}} |
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | * '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | ||
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=References= | =References= | ||
| − | <pubmed>10844689,12718886,11250911,,16479537, 10844689 19744498 19968790 </pubmed> | + | <pubmed>10844689,12718886,11250911, 20122408,16479537, 10844689 19744498 19968790 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:22, 4 February 2010
- Description: replicative DNA helicase, part of the replisome
| Gene name | dnaC |
| Synonyms | |
| Essential | yes PubMed |
| Product | replicative DNA helicase |
| Function | DNA replication |
| MW, pI | 50 kDa, 4.548 |
| Gene length, protein length | 1362 bp, 454 aa |
| Immediate neighbours | yycE, yycD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU40440
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: DnaB subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: DnaB-DnaC PubMed, DnaI-DnaC PubMed, part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- UniProt: P37469
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790]
[WorldCat.org]
[DOI]
(I p)
Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498]
[WorldCat.org]
[DOI]
(I p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886]
[WorldCat.org]
[DOI]
(P p)
M Bárcena, T Ruiz, L E Donate, S E Brown, N E Dixon, M Radermacher, J M Carazo
The DnaB.DnaC complex: a structure based on dimers assembled around an occluded channel.
EMBO J: 2001, 20(6);1462-8
[PubMed:11250911]
[WorldCat.org]
[DOI]
(P p)
Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689]
[WorldCat.org]
[DOI]
(P p)
