Difference between revisions of "RpoE"
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=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' | |
| − | * '''Operon:''' ''[[fadF]]-[[acdA]]-[[rpoE]]'' | + | ** ''[[fadF]]-[[acdA]]-[[rpoE]]'' {{PubMed|17189250}} |
| + | ** ''[[rpoE]]'' {{PubMed|10336502}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| + | ** ''[[fadF]]'': [[SigA]] {{PubMed|17189250}} | ||
| + | ** ''[[rpoE]]'': [[SigA]] {{PubMed|10336502}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** ''[[fadF]]'': repressed in the absence of long-chain fatty acids ([[FadR]]) | + | ** ''[[fadF]]'': repressed in the absence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[FadR]]: transcription repression | + | ** [[FadR]]: transcription repression {{PubMed|17189250}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:23, 3 February 2010
- Description: RNA polymerase delta subunit
| Gene name | rpoE |
| Synonyms | |
| Essential | no |
| Product | RNA polymerase delta subunit |
| Function | transcription |
| MW, pI | 20 kDa, 3.654 |
| Gene length, protein length | 519 bp, 173 aa |
| Immediate neighbours | pyrG, acdA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU37160
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: rpoE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P12464
- KEGG entry: [3]
- E.C. number: 2.7.7.6
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Your additional remarks
References
Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250]
[WorldCat.org]
[DOI]
(P p)
F J López de Saro, N Yoshikawa, J D Helmann
Expression, abundance, and RNA polymerase binding properties of the delta factor of Bacillus subtilis.
J Biol Chem: 1999, 274(22);15953-8
[PubMed:10336502]
[WorldCat.org]
[DOI]
(P p)
F J López de Saro, A Y Woody, J D Helmann
Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase.
J Mol Biol: 1995, 252(2);189-202
[PubMed:7545758]
[WorldCat.org]
[DOI]
(P p)
Y L Juang, J D Helmann
Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: effects of temperature, delta protein, and sigma factor mutations.
Biochemistry: 1995, 34(26);8465-73
[PubMed:7599136]
[WorldCat.org]
[DOI]
(P p)
Y L Juang, J D Helmann
The delta subunit of Bacillus subtilis RNA polymerase. An allosteric effector of the initiation and core-recycling phases of transcription.
J Mol Biol: 1994, 239(1);1-14
[PubMed:7515111]
[WorldCat.org]
[DOI]
(P p)
M Lampe, C Binnie, R Schmidt, R Losick
Cloned gene encoding the delta subunit of Bacillus subtilis RNA polymerase.
Gene: 1988, 67(1);13-9
[PubMed:2843435]
[WorldCat.org]
[DOI]
(P p)
