Difference between revisions of "FabI"

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(Expression and regulation)
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* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:'''  
 +
** inhibited by triclosan {{PubMed|11325930}}
  
 
* '''Interactions:'''
 
* '''Interactions:'''
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* '''Regulation:'''  
 
* '''Regulation:'''  
 
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
 
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
** inhibited by triclosan {{PubMed|11325930}}
 
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 14:17, 14 January 2010

  • Description: enoyl-acyl carrier protein reductase

Gene name fabI
Synonyms yjbW
Essential no
Product enoyl-acyl carrier protein reductase
Function fatty acid biosynthesis
MW, pI 27 kDa, 5.605
Gene length, protein length 774 bp, 258 aa
Immediate neighbours thiD, cotO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FabI context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)
  • Protein family: FabI subfamily (according to Swiss-Prot)
  • Paralogous protein(s): FabL, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • inhibited by triclosan PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure: 1DFI (the enzyme from E. coli in complex with NAD) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications