Difference between revisions of "GanB"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
* '''Operon:''' ''[[yvfK]]-[[yvfL]]-[[yvfM]]-[[lacA]]-[[yvfO]]'' {{PubMed|9287030}}  
+
* '''Operon:''' ''[[cycB]]-[[yvfL]]-[[yvfM]]-[[lacA]]-[[yvfO]]'' {{PubMed|9287030}}  
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
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** [[LacR]]: transcription repression {{PubMed|9287030}}  
 
** [[LacR]]: transcription repression {{PubMed|9287030}}  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 11:17, 29 December 2009

  • Description: arabinogalactan endo-1,4-beta-galactosidase

Gene name yvfO
Synonyms
Essential no
Product arabinogalactan endo-1,4-beta-galactosidase
Function galactan utilization
MW, pI 47 kDa, 8.243
Gene length, protein length 1287 bp, 429 aa
Immediate neighbours rsbP, lacA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvfO context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU34120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-galactosidic linkages in arabinogalactans (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 53 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2J74 (B. licheniformis)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)