Difference between revisions of "CadA"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''cadA'' {{PubMed|12779235}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12779235}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed in the absence of toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) ([[CzrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15948947 PubMed]
+
** induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) ([[CzrA]]) {{PubMed|12779235,15948947}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[CzrA]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15948947 PubMed]
+
** [[CzrA]]:  transcription repression {{PubMed|15948947}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 08:34, 29 December 2009

  • Description: cadmium transporting ATPase, resistance to cadmium

Gene name cadA
Synonyms yvgW
Essential no
Product cadmium transporting ATPase
Function cadmium export
MW, pI 75 kDa, 5.998
Gene length, protein length 2106 bp, 702 aa
Immediate neighbours bdbD, copA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvgW context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU33490

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out) (according to Swiss-Prot)
  • Protein family: Type IB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Charles M Moore, Ahmed Gaballa, Monica Hui, Rick W Ye, John D Helmann
Genetic and physiological responses of Bacillus subtilis to metal ion stress.
Mol Microbiol: 2005, 57(1);27-40
[PubMed:15948947] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

I M Solovieva, K D Entian
Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd(2+) ion resistance.
FEMS Microbiol Lett: 2002, 208(1);105-9
[PubMed:11934502] [WorldCat.org] [DOI] (P p)