Difference between revisions of "LeuS"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[leuS]]'' {{PubMed|1317842}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by leucine limitation ([[T-box]]) {{PubMed|1317842}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[T-box]]: RNA switch, binding of uncharged  tRNA-Leu results in transcription antitermination {{PubMed|1317842}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=References=
 
=References=
 
+
==Reviews==
 
<pubmed>19258532,, </pubmed>
 
<pubmed>19258532,, </pubmed>
 +
==Original Publications==
 +
<pubmed>1317842,, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:52, 19 December 2009

  • Description: Leu-tRNA synthetase

Gene name leuS
Synonyms
Essential yes PubMed
Product leucyl-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 91 kDa, 4.857
Gene length, protein length 2412 bp, 804 aa
Immediate neighbours ytwF, ytvB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LeuS context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU30320

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • T-box: RNA switch, binding of uncharged tRNA-Leu results in transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842] [WorldCat.org] [DOI] (P p)