Difference between revisions of "WalK"

From SubtiWiki
Jump to: navigation, search
(Basic information/ Evolution)
Line 54: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[WalR]]
  
* '''Protein family:'''
+
* '''Protein family:''' two-component sensor kinase of the OmpR family
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''

Revision as of 19:30, 30 November 2009

  • Description: two-component sensor kinase, controls cell wall metabolism

Gene name walK
Synonyms yycG
Essential yes PubMed
Product two-component sensor kinase (OmpR family)
Function control of cell wall metabolism
MW, pI 69 kDa, 4.775
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours yycH, walR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YycG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU40400

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of WalR
  • Protein family: two-component sensor kinase of the OmpR family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), part of the division septum in growing cells PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Sarah Dubrac, Paola Bisicchia, Kevin M Devine, Tarek Msadek
A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway.
Mol Microbiol: 2008, 70(6);1307-22
[PubMed:19019149] [WorldCat.org] [DOI] (I p)

Sarah Dubrac, Tarek Msadek
Tearing down the wall: peptidoglycan metabolism and the WalK/WalR (YycG/YycF) essential two-component system.
Adv Exp Med Biol: 2008, 631;214-28
[PubMed:18792692] [WorldCat.org] [DOI] (P p)

Original Publications