Difference between revisions of "CwlH"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' [[cwlH]] {{PubMed|10515909}} | + | * '''Operon:''' ''[[cwlH]]'' {{PubMed|10515909}} |
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|10515909}} | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|10515909}} | ||
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** [[GerE]]: transcription activation {{PubMed|10515909}} | ** [[GerE]]: transcription activation {{PubMed|10515909}} | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 19:47, 24 November 2009
- Description: N-acetylmuramoyl-L-alanine amidase
| Gene name | cwlH |
| Synonyms | yqeE |
| Essential | no |
| Product | N-acetylmuramoyl-L-alanine amidase |
| Function | cell wall metabolism |
| MW, pI | 27 kDa, 9.998 |
| Gene length, protein length | 750 bp, 250 aa |
| Immediate neighbours | yqeF, yqeD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU25710
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
- Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54450
- KEGG entry: [3]
- E.C. number: 3.5.1.28
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275]
[WorldCat.org]
[DOI]
(P p)
F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909]
[WorldCat.org]
[DOI]
(P p)
