Difference between revisions of "SpoIIIE"

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(References)
(Basic information/ Evolution)
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes {{PubMed|19818024}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''

Revision as of 08:34, 13 October 2009

  • Description: ATP-dependent DNA translocase

Gene name spoIIIE
Synonyms
Essential no
Product ATP-dependent DNA translocase required for chromosome

partitioning through the septum into the forespore

Function chromosome partition during sporulation
MW, pI 86 kDa, 6.757
Gene length, protein length 2361 bp, 787 aa
Immediate neighbours tepA, ymfC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIIE context.gif
This image was kindly provided by SubtiList






The gene

Basic information

  • Locus tag: BSU16800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • Protein family:
  • Paralogous protein(s): SftA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christine Kaimer, José Eduardo González-Pastor, Peter L Graumann
SpoIIIE and a novel type of DNA translocase, SftA, couple chromosome segregation with cell division in Bacillus subtilis.
Mol Microbiol: 2009, 74(4);810-25
[PubMed:19818024] [WorldCat.org] [DOI] (I p)

Kathleen A Marquis, Briana M Burton, Marcelo Nollmann, Jerod L Ptacin, Carlos Bustamante, Sigal Ben-Yehuda, David Z Rudner
SpoIIIE strips proteins off the DNA during chromosome translocation.
Genes Dev: 2008, 22(13);1786-95
[PubMed:18593879] [WorldCat.org] [DOI] (P p)

Briana M Burton, Kathleen A Marquis, Nora L Sullivan, Tom A Rapoport, David Z Rudner
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell: 2007, 131(7);1301-12
[PubMed:18160039] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
Assembly of the SpoIIIE DNA translocase depends on chromosome trapping in Bacillus subtilis.
Curr Biol: 2003, 13(24);2196-200
[PubMed:14680637] [WorldCat.org] (P p)

J Bath, L J Wu, J Errington, J C Wang
Role of Bacillus subtilis SpoIIIE in DNA transport across the mother cell-prespore division septum.
Science: 2000, 290(5493);995-7
[PubMed:11062134] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Use of asymmetric cell division and spoIIIE mutants to probe chromosome orientation and organization in Bacillus subtilis.
Mol Microbiol: 1998, 27(4);777-86
[PubMed:9515703] [WorldCat.org] [DOI] (P p)

K Pogliano, A E Hofmeister, R Losick
Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1997, 179(10);3331-41
[PubMed:9150232] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Septal localization of the SpoIIIE chromosome partitioning protein in Bacillus subtilis.
EMBO J: 1997, 16(8);2161-9
[PubMed:9155041] [WorldCat.org] [DOI] (P p)

M E Sharpe, J Errington
Postseptational chromosome partitioning in bacteria.
Proc Natl Acad Sci U S A: 1995, 92(19);8630-4
[PubMed:7567988] [WorldCat.org] [DOI] (P p)

L J Wu, P J Lewis, R Allmansberger, P M Hauser, J Errington
A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.
Genes Dev: 1995, 9(11);1316-26
[PubMed:7797072] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division.
Science: 1994, 264(5158);572-5
[PubMed:8160014] [WorldCat.org] [DOI] (P p)

D Foulger, J Errington
The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1247-55
[PubMed:2507870] [WorldCat.org] [DOI] (P p)

S Panzer, R Losick, D Sun, P Setlow
Evidence for an additional temporal class of gene expression in the forespore compartment of sporulating Bacillus subtilis.
J Bacteriol: 1989, 171(1);561-4
[PubMed:2492502] [WorldCat.org] [DOI] (P p)