Difference between revisions of "PheS"
| Line 92: | Line 92: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[pheS]]-[[pheT]]'' {{PubMed|2127701}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2127701}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced by phenylalanine limitation ([[T-box]]) {{PubMed|19258532}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 122: | Line 124: | ||
=References= | =References= | ||
| − | <pubmed>19258532,, </pubmed> | + | <pubmed>19258532,2127701 , </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:04, 1 October 2009
- Description: phenylalanyl-tRNA synthetase (alpha subunit)
| Gene name | pheS |
| Synonyms | |
| Essential | yes PubMed |
| Product | phenylalanyl-tRNA synthetase (alpha subunit) |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 38 kDa, 5.083 |
| Gene length, protein length | 1032 bp, 344 aa |
| Immediate neighbours | pheT, ysgA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28640
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
- Protein family: Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P17921
- KEGG entry: [3]
- E.C. number: 6.1.1.20
Additional information
Expression and regulation
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701]
[WorldCat.org]
[DOI]
(P p)
