Difference between revisions of "PtsI"
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| − | * '''Description:''' Enzyme I, general (non sugar-specific) component of the PTS. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein ([[PtsH |HPr]]) <br/><br/> | + | * '''Description:''' Enzyme I, general (non sugar-specific) component of the [[PTS]]. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein ([[PtsH |HPr]]) <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase system (PTS) enzyme I | + | |style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase system ([[PTS]]) enzyme I |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || PTS-dependent sugar transport | + | |style="background:#ABCDEF;" align="center"|'''Function''' || [[PTS]]-dependent sugar transport |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | ||
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**pyrophosphate/phosphate carrier histidine (central Domain) | **pyrophosphate/phosphate carrier histidine (central Domain) | ||
| − | * '''Modification:''' transient autophosphorylation on His-189 | + | * '''Modification:''' |
| + | ** transient autophosphorylation on His-189 | ||
| + | ** ''in vivo'' also phosphorylated on Ser-34 or Ser-36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed] | ||
* '''Cofactor(s):''' Magnesium | * '''Cofactor(s):''' Magnesium | ||
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* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' cytoplasm (according to Swiss-Prot) | + | * '''Localization:''' cytoplasm (according to Swiss-Prot) |
=== Database entries === | === Database entries === | ||
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11902727 PubMed] | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11902727 PubMed] | ||
| − | |||
| − | * '''Regulatory mechanism:''' ''[[ptsG]]'': transcriptional antitermination via the [[GlcT]]-dependent RNA-switch | + | * '''Regulation:''' |
| + | ** expression activated by glucose (4.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed], induction by glucose (''[[ptsG]]''), constitutive (''[[ptsH]]'') | ||
| + | |||
| + | * '''Regulatory mechanism:''' | ||
| + | ** ''[[ptsG]]'': transcriptional antitermination via the [[GlcT]]-dependent RNA-switch | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:45, 20 September 2009
- Description: Enzyme I, general (non sugar-specific) component of the PTS. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)
| Gene name | ptsI |
| Synonyms | |
| Essential | no |
| Product | phosphotransferase system (PTS) enzyme I |
| Function | PTS-dependent sugar transport |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 62,9 kDa, 4.59 |
| Gene length, protein length | 1710 bp, 570 amino acids |
| Immediate neighbours | ptsH, splA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU13910
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine (according to Swiss-Prot) PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to HPr (His-15)
- Protein family: PEP-utilizing enzyme family (according to Swiss-Prot) PEP-utilizing enzyme family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- HPr binding site (N-Terminal Domain)
- pyruvate binding site (C-Terminal Domain)
- pyrophosphate/phosphate carrier histidine (central Domain)
- Modification:
- transient autophosphorylation on His-189
- in vivo also phosphorylated on Ser-34 or Ser-36 PubMed
- Cofactor(s): Magnesium
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 2HWG (Enzyme I from E. coli)
- UniProt: P08838
- KEGG entry: [3]
- E.C. number: 2.7.3.9 2.7.3.9]
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant: GP864 (ermC), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Josef Deutscher, Paris-Grignon, France
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
