Difference between revisions of "HemY"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[hemE]]-[[hemH]]-[[hemY]]'' {{PubMed|1459957}} |
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 19:50, 16 September 2009
- Description: protoporphyrinogen IX oxidase
| Gene name | hemY |
| Synonyms | hemG |
| Essential | no |
| Product | protoporphyrinogen IX oxidase |
| Function | heme biosynthesis |
| MW, pI | 51 kDa, 8.042 |
| Gene length, protein length | 1410 bp, 470 aa |
| Immediate neighbours | hemH, yhgD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU10140
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2 (according to Swiss-Prot)
- Protein family: protoporphyrinogen oxidase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P32397
- KEGG entry: [3]
- E.C. number: 1.3.3.4
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lu Sun, Xin Wen, Ying Tan, Heyang Li, Xing Yang, Yuefang Zhao, Baifan Wang, Qiongyao Cao, Congwei Niu, Zhen Xi
Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase.
Amino Acids: 2009, 37(3);523-30
[PubMed:19266155]
[WorldCat.org]
[DOI]
(I p)
M Hansson, M C Gustafsson, C G Kannangara, L Hederstedt
Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity.
Biochim Biophys Acta: 1997, 1340(1);97-104
[PubMed:9217019]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX.
J Bacteriol: 1994, 176(19);5962-70
[PubMed:7928957]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957]
[WorldCat.org]
[DOI]
(P p)
