Difference between revisions of "GlnR"
| Line 94: | Line 94: | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' expressed in the absence of glutamine [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed] | + | * '''Regulation:''' |
| + | ** expressed in the absence of glutamine ([[GlnR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed] | ||
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| + | ** [[GlnR]]-[[GlnA]] complex: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed] | ||
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ||
| Line 108: | Line 110: | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | * '''lacZ fusion:''' | + | * '''lacZ fusion:''' pGP189 (in pAC7), available in [[Stülke]] lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
Revision as of 17:40, 1 September 2009
| Gene name | glnR |
| Synonyms | |
| Essential | no |
| Product | transcription repressor |
| Function | regulation of glutamine synthesis |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cell wall | |
| MW, pI | 15 kDa, 9.731 |
| Gene length, protein length | 405 bp, 135 aa |
| Immediate neighbours | ynbB, glnA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17450
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): feedback-inhibited GlnA
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P37582
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion: pGP189 (in pAC7), available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
