Difference between revisions of "GlnR"

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* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' expressed in the absence of glutamine [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
+
* '''Regulation:'''  
 +
** expressed in the absence of glutamine ([[GlnR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
 
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
 
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
  
* '''Regulatory mechanism:''' repressed by [[GlnR]]-[[GlnA]] complex [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
+
* '''Regulatory mechanism:'''  
 +
** [[GlnR]]-[[GlnA]] complex: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
 
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
 
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
  
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP189 (in pAC7), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 17:40, 1 September 2009

  • Description: transcriptional repressor of the glnR-glnA operon

Gene name glnR
Synonyms
Essential no
Product transcription repressor
Function regulation of glutamine synthesis
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate, Cell wall
MW, pI 15 kDa, 9.731
Gene length, protein length 405 bp, 135 aa
Immediate neighbours ynbB, glnA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlnR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU17450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): feedback-inhibited GlnA
  • Effectors of protein activity:
  • Interactions: GlnR-GlnA, with feedback-inhibited GlnA, this results in DNA binding
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of glutamine (GlnR) PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP189 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References