Sda

• Description: developmental checkpoint protein, inhibits the autokinase activity of KinA

Gene name	sda
Synonyms
Essential	no
Product	developmental checkpoint protein
Function	mediates a developmental checkpoint coupling initiation of sporulation (phosphorylation of Spo0A) to the function of replication initiation proteins
MW, pI	6 kDa, 5.605
Gene length, protein length	156 bp, 52 aa
Immediate neighbours	yloV, sdaAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25690

Phenotypes of a mutant

• sporulates at a higher frequency
• will sporulate in the presence of replication stress

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: KinB-Sda, KinA-Sda

• Localization:

Database entries

• Structure: 3D36 (from Geobacillus stearothermophilus, complex with KinB), 1PV0

• UniProt: Q7WY62

• KEGG entry: [3]

• E.C. number:

Additional information

Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Expression and regulation

• Operon: sda

• Sigma factor: SigA

• Regulation: DnaA
  • repressed when no DNA damage is present (LexA) PubMed

• Regulatory mechanism: Probably activated by DnaA under replication stress
  • LexA: transcription repression PubMed

• Additional information: Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Bill Burkholder Homepage

References

Jan-Willem Veening, Heath Murray, Jeff Errington
A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis.
Genes Dev: 2009, 23(16);1959-70
[PubMed:19684115] [WorldCat.org] [DOI] (I p)

David A Jacques, Margaret Streamer, Susan L Rowland, Glenn F King, J Mitchell Guss, Jill Trewhella, David B Langley
Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state.
Acta Crystallogr D Biol Crystallogr: 2009, 65(Pt 6);574-81
[PubMed:19465772] [WorldCat.org] [DOI] (I p)

Michael V Ruvolo, Kathleen E Mach, William F Burkholder
Proteolysis of the replication checkpoint protein Sda is necessary for the efficient initiation of sporulation after transient replication stress in Bacillus subtilis.
Mol Microbiol: 2006, 60(6);1490-508
[PubMed:16796683] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Susan L Rowland, William F Burkholder, Katherine A Cunningham, Mark W Maciejewski, Alan D Grossman, Glenn F King
Structure and mechanism of action of Sda, an inhibitor of the histidine kinases that regulate initiation of sporulation in Bacillus subtilis.
Mol Cell: 2004, 13(5);689-701
[PubMed:15023339] [WorldCat.org] [DOI] (P p)

W F Burkholder, I Kurtser, A D Grossman
Replication initiation proteins regulate a developmental checkpoint in Bacillus subtilis.
Cell: 2001, 104(2);269-79
[PubMed:11207367] [WorldCat.org] [DOI] (P p)