Difference between revisions of "NadE"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''nadE'' {{PubMed|2435704}} |
| − | * '''[[Sigma factor]]:''' [[SigB]] | + | * '''[[Sigma factor]]:''' [[SigA]], [[SigB]] {{PubMed|9271869,11544224}} |
| − | * '''Regulation:''' induced by stress ([[SigB]]) | + | * '''Regulation:''' induced by stress ([[SigB]]) {{PubMed|11544224}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 20:08, 5 August 2009
- Description: NH3-dependent NAD+ synthetase
| Gene name | nadE |
| Synonyms | outB, tscBGH, gsp-81 |
| Essential | yes PubMed |
| Product | NH3-dependent NAD+ synthetase |
| Function | NAD biosynthesis |
| MW, pI | 30 kDa, 4.888 |
| Gene length, protein length | 816 bp, 272 aa |
| Immediate neighbours | ycgI, tmrB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU03130
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ (according to Swiss-Prot)
- Protein family: NAD synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1KQP
- UniProt: P08164
- KEGG entry: [3]
- E.C. number: 6.3.1.5
Additional information
Expression and regulation
- Operon: nadE PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
