UxuA

• Description: D-mannonate hydrolyase
  
  

Gene name	uxuA
Synonyms	yjmE
Essential	no
Product	D-mannonate hydrolyase
Function	hexuronate utilization
MW, pI	40 kDa, 4.92
Gene length, protein length	1077 bp, 359 aa
Immediate neighbours	yjmD, yjmF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU12340

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H₂O (according to Swiss-Prot)

• Protein family: mannonate dehydratase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34346

• KEGG entry: [3]

• E.C. number: 4.2.1.8

Expression and regulation

• Operon:
  • uxaC-yjmB-yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA PubMed
  • yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA PubMed

• Sigma factor: SigE PubMed
  • uxaC: SigA PubMed
  • yjmC: SigE PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed1 PubMed2
  • induced by galacturonate (ExuR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed1 PubMed2
  • ExuR: reanscription repression in the absence of inducer PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: