Difference between revisions of "YsxC"

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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SVI 1SVI] (complex with GDP),  [http://www.rcsb.org/pdb/explore.do?structureId=1SUL 1SUL]
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SVI 1SVI] (complex with GDP),  [http://www.rcsb.org/pdb/explore.do?structureId=1SUL 1SUL]
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38424 P38424]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P38424 P38424]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28190]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28190]

Revision as of 13:56, 20 July 2009

  • Description: assembly of the 50S subunit of the ribosome

Gene name ysxC
Synonyms orfX
Essential yes PubMed
Product GTPase
Function assembly of the 50S subunit of the ribosome
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 21 kDa, 9.708
Gene length, protein length 585 bp, 195 aa
Immediate neighbours ysxD, lonA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YsxC context.gif







The gene

Basic information

  • Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: engB family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1SVI (complex with GDP), 1SUL
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP834 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References