Difference between revisions of "Ogt"
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 495 bp, 165 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 495 bp, 165 aa | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kinE]]'', ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kinE]]'', ''[[mtnA]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13227]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13227]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | ||
Revision as of 19:45, 15 July 2009
- Description: O6-methylguanine DNA alkyltransferase
| Gene name | ogt |
| Synonyms | dat |
| Essential | no |
| Product | O6-methylguanine DNA alkyltransferase |
| Function | DNA repair |
| MW, pI | 18 kDa, 7.092 |
| Gene length, protein length | 495 bp, 165 aa |
| Immediate neighbours | kinE, mtnA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU13540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)
- Protein family: MGMT family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P11742
- KEGG entry: [3]
- E.C. number: 2.1.1.63
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
J A Rafferty, J E Wibley, P Speers, I Hickson, G P Margison, P C Moody, K T Douglas
The potential role of glycine-160 of human O6-alkylguanine-DNA alkyltransferase in reaction with O6-benzylguanine as determined by site-directed mutagenesis and molecular modelling comparisons.
Biochim Biophys Acta: 1997, 1342(1);90-102
[PubMed:9366274]
[WorldCat.org]
[DOI]
(P p)
F Morohoshi, N Munakata
Isolation of a Bacillus subtilis mutant defective in constitutive O6-alkylguanine-DNA alkyltransferase.
Mutat Res: 1990, 235(1);15-23
[PubMed:2105461]
[WorldCat.org]
[DOI]
(P p)
F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA alkyltransferase.
Nucleic Acids Res: 1989, 17(16);6531-43
[PubMed:2506524]
[WorldCat.org]
[DOI]
(P p)
