Difference between revisions of "LytC"
(→Phenotypes of a mutant) |
|||
| Line 1: | Line 1: | ||
| − | * '''Description:''' N-acetylmuramoyl-L-alanine amidase <br/><br/> | + | * '''Description:''' N-acetylmuramoyl-L-alanine amidase, required for flagellar function [http://www.ncbi.nlm.nih.gov/sites/entrez/19542270 PubMed]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
Revision as of 06:00, 25 June 2009
- Description: N-acetylmuramoyl-L-alanine amidase, required for flagellar function PubMed
| Gene name | lytC |
| Synonyms | cwlB |
| Essential | no |
| Product | N-acetylmuramoyl-L-alanine amidase
(EC 3.5.1.28) |
| Function | major autolysin, cell separation, wall turnover
lysis, motility and general cell lysis induced by sodium azide |
| MW, pI | 52 kDa, 10.108 |
| Gene length, protein length | 1488 bp, 496 aa |
| Immediate neighbours | tuaA, lytB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU35620
Phenotypes of a mutant
impaired in motility, the phenotype is suppressed by mutations in lonA or smiA PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
- Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: Q02114
- KEGG entry: [3]
- E.C. number: 3.5.1.28
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270]
[WorldCat.org]
[DOI]
(I p)
Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841]
[WorldCat.org]
[DOI]
(P p)
T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275]
[WorldCat.org]
[DOI]
(P p)
