YomL - Revision history

134.76.38.147 at 15:22, 2 April 2014

2014-04-02T15:22:48Z

Jstuelk at 17:31, 13 November 2013

2013-11-13T17:31:30Z

Jstuelk at 11:52, 2 November 2013

2013-11-02T11:52:41Z

Fabian Commichau: /* Biological materials */

2013-09-30T19:14:31Z

‎Biological materials

Raphael2215 at 13:55, 26 August 2013

2013-08-26T13:55:52Z

Fmehne: /* Biological materials */

2013-08-13T16:35:56Z

‎Biological materials

134.76.70.252 at 13:40, 16 May 2013

2013-05-16T13:40:03Z

134.76.70.252 at 10:29, 14 May 2013

2013-05-14T10:29:58Z

134.76.70.252 at 13:05, 13 May 2013

2013-05-13T13:05:02Z

Kgunka: /* Biological materials */

2013-05-08T14:08:05Z

‎Biological materials

← Older revision		Revision as of 15:22, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' unknown protein<br/><br/>~~	+	{{#bank: YomL}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''yomL''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no {{PubMed\|24178028}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| unknown~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| unknown~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21320 yomL]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 25 kDa, 9.357~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 684 bp, 228 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yozP]]'', ''[[youB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU21320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU21320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU21320 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yomL_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[proteins of unknown function]]}},~~
−	~~{{SubtiWiki category\|[[SPß prophage]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU21320
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13599]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[YddT]] (the two proteins differ in only 4 out of 228 amino acids)
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O31973 O31973]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU21320]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=BSU21320 yomL] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	**BP118 (<i>yomL::kan</i>), available in [[Fabian Commichau]]'s lab {{PubMed\|24178028}}
−
−	* '''Expression vector:'''
−	** IPTG inducible expression of Strep-''yomL'' in ''E. coli'': pGP2580 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>17114254 23420519 24178028 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 8: / Line 8: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no {{PubMed|24178028}}
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || unknown

@@ Line 1: / Line 1: @@
-* '''Description:''' unknown essential protein<br/><br/>
+* '''Description:''' unknown protein<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 8: / Line 8: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|17114254}}
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || unknown
@@ Line 38: / Line 38: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[proteins of unknown function]]}},
 {{SubtiWiki category|[[SPß prophage]]}}
@@ Line 51: / Line 50: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 117: / Line 115: @@
 * '''Mutant:'''
-**BP118 (<i>yomL::kan</i>), available in [[Fabian Commichau]]'s lab
+**BP118 (<i>yomL::kan</i>), available in [[Fabian Commichau]]'s lab {{PubMed|24178028}}
 * '''Expression vector:'''
@@ Line 135: / Line 133: @@
 =References=
-<pubmed>17114254  23420519 </pubmed>
+<pubmed>17114254  23420519 24178028 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 117: / Line 117: @@
 * '''Mutant:'''
-**BP118 (kan), available in [[Fabian Commichau]]'s lab
+**BP118 (<i>yomL::kan</i>), available in [[Fabian Commichau]]'s lab
 * '''Expression vector:'''

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=BSU21320 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yomL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21320]]
 |}

← Older revision		Revision as of 16:35, 13 August 2013
Line 121:		Line 121:

	* '''Expression vector:'''		* '''Expression vector:'''
		+	** IPTG inducible expression of Strep-''yomL'' in ''E. coli'': pGP2580 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yozP]]'', ''[[youB]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU21320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU21320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU21320 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU21320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU21320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU21320 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yomL_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU21320 yomL]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21320 yomL]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.357
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yozP]]'', ''[[youB]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14050&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU21320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU21320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU21320 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yomL_context.gif]]

← Older revision		Revision as of 14:08, 8 May 2013
Line 118:		Line 118:

	* '''Mutant:'''		* '''Mutant:'''
		+	**BP118 (kan), available in [[Fabian Commichau]]'s lab

	* '''Expression vector:'''		* '''Expression vector:'''