Testing - Revision history

Lflorez at 14:43, 16 July 2009

2009-07-16T14:43:08Z

Robot at 14:41, 16 July 2009

2009-07-16T14:41:44Z

Lflorez at 14:35, 16 July 2009

2009-07-16T14:35:43Z

Lflorez at 14:35, 16 July 2009

2009-07-16T14:35:34Z

Robot: Replaced content with 'Testing, 1, 2, 3'

2009-07-16T14:34:09Z

Replaced content with 'Testing, 1, 2, 3'

Lflorez: Created page with '* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme

{| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name…'

2009-07-06T07:30:19Z

Created page with '* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name…'

New page

* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''eno''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enolase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46,4 kDa, 4.49
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1290 bp, 430 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pgm]]'', ''[[yvgK]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:eno_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33900

===Phenotypes of a mutant ===

* Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

* '''Protein family:''' enolase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Domains:'''
** substrate binding domain (366–369)

* '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]

* '''Cofactor(s):''' Mg2+

* '''Effectors of protein activity:'''
** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Interactions:''' [[Eno]]-[[PfkA]], [[Eno]]-[[Rny]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]

* '''Localization:''' cytoplasm (according to Swiss-Prot), cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] and membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3ES8 3ES8] (from ''Oceanobacillus iheyensis'', complex with Mg(2+) and malate)

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37869 P37869]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]

=== Additional information===
There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''

* '''Sigma factor:''' [[SigA]]

* '''Regulation:''' expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
**''[[cggR]]'': neg. regulated by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed], induced by sugar
**''[[pgk]]'': constitutive [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]

* '''Regulatory mechanism:''' transcription repression by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:''' pGP563 (N-terminal His-tag, in [[pWH844]]), pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=

<pubmed> 17726680, 17218307, 12850135 , 19193632 , 11489127, 8021172, 17505547, 25885 </pubmed>

← Older revision		Revision as of 14:43, 16 July 2009
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−	Testing, 1, 2, 3	+	Testing, 1, 2, 3, 5

← Older revision		Revision as of 14:41, 16 July 2009
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−	Testing, 1, 2, 3~~, 4, 5~~	+	Testing, 1, 2, 3

← Older revision		Revision as of 14:35, 16 July 2009
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−	Testing, 1, 2, 3, 4	+	Testing, 1, 2, 3, 4, 5

← Older revision		Revision as of 14:35, 16 July 2009
Line 1:		Line 1:
−	Testing, 1, 2, 3	+	Testing, 1, 2, 3, 4

← Older revision		Revision as of 14:34, 16 July 2009
Line 1:		Line 1:
−	* '''Description:''' enolase, ~~glycolytic/ gluconeogenic enzyme<br/><br/>~~	+	Testing, 1, 2, 3
−
−	~~{\| align="right" border="~~1~~" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''eno''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| enolase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| enzyme in glycolysis/ gluconeogenesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 46,4 kDa~~, ~~4.49~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1290 bp, 430 amino acids~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[pgm]]'', ''[[yvgK]]''~~
−	\|-
−	~~\|colspan="~~2~~" style="background:#FAF8CC;" align="center"\|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] <br/> (Barbe ''et al.''~~, ~~2009)'''~~
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:eno_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|}
−
−	~~__TOC__~~
−
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU33900
−
−	~~===Phenotypes of a mutant ===~~
−
−	* Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H<sub>2</sub>O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
−
−	* '''Protein family:''' enolase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
−
−	* '''Domains:'''
−	** substrate binding domain (366–369)
−
−	* '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
−
−	* '''Cofactor(s):''' Mg2+
−
−	* '''Effectors of protein activity:'''
−	** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
−
−	* '''Interactions:''' [[Eno]]-[[PfkA]], [[Eno]]-[[Rny]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
−
−	* '''Localization:''' cytoplasm (according to Swiss-Prot), cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] and membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed]
−
−	~~=== Database entries ===~~
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3ES8 3ES8] (from ''Oceanobacillus iheyensis'', complex with Mg(2+) and malate)
−
−	* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37869 P37869]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]
−
−	~~=== Additional information===~~
−	~~There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−	** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
−	** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
−
−	* '''Sigma factor:''' [[SigA]]
−
−	* '''Regulation:''' expression activated by glucose (3~~.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]~~
−	**''[[cggR]]'': neg. regulated by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed], induced by sugar
−	**''[[pgk]]'': constitutive [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
−
−	* '''Regulatory mechanism:''' transcription repression by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:''' pGP563 (N-terminal His-tag, in [[pWH844]]), pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
−
−	* '''Antibody:''' available in [[Stülke]] lab
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Stülke\|Jörg Stülke]], University of Göttingen, Germany~~
−	~~[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed> 17726680, 17218307, 12850135 , 19193632 , 11489127, 8021172, 17505547, 25885 </pubmed>~~

Testing - Revision history

Lflorez at 14:43, 16 July 2009

Robot at 14:41, 16 July 2009

Lflorez at 14:35, 16 July 2009

Lflorez at 14:35, 16 July 2009

Robot: Replaced content with 'Testing, 1, 2, 3'

Lflorez: Created page with '* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name…'

Lflorez: Created page with '* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme

{| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name…'