SpsA - Revision history

Jstuelk: /* Expression and regulation */

2015-04-06T14:10:50Z

‎Expression and regulation

Jstuelk at 11:19, 23 September 2014

2014-09-23T11:19:19Z

Jstuelk at 11:01, 23 September 2014

2014-09-23T11:01:24Z

Jstuelk: /* References */

2014-09-23T10:50:38Z

‎References

Jstuelk at 10:49, 23 September 2014

2014-09-23T10:49:52Z

Jstuelk at 10:49, 23 September 2014

2014-09-23T10:49:13Z

Jstuelk at 10:46, 23 September 2014

2014-09-23T10:46:27Z

134.76.38.147 at 15:06, 2 April 2014

2014-04-02T15:06:49Z

134.76.70.252 at 14:52, 16 May 2013

2013-05-16T14:52:20Z

134.76.70.252 at 11:34, 14 May 2013

2013-05-14T11:34:48Z

← Older revision		Revision as of 14:10, 6 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' nucleotide-sugar-dependent glycosyltransferase, spore coat polysaccharide synthesis <br/><br/>~~	+	{{#bank: SpsA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''spsA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ipa-63d ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| nucleotide-sugar-dependent glycosyltransferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| spore coat polysaccharide synthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37910 spsA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 30 kDa, 5.358~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 768 bp, 256 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[spsB]]'', ''[[gerQ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37910 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:spsA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsA_3892351_3893121_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:spsA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37910]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GerE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigK regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU37910
−
−	~~===Phenotypes of a mutant ===~~
−	* reduced [[germination]] efficiency {{PubMed\|25239894}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37910&redirect=T BSU37910]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sps.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10609]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' glycosyltransferase 2 family {{PubMed\|10350455}}
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37910&redirect=T BSU37910]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore.do?structureId=1QGS 1QGS] (complex with UDP) {{PubMed\|10350455}}
−	** [http://www.rcsb.org/pdb/explore.do?structureId=1H7Q 1H7Q] {{PubMed\|11733986}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39621 P39621]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37910]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[spsA]]-[[spsB]]-[[spsC]]-[[spsD]]-[[spsF]]-[[spsG]]-[[spsI]]-[[spsJ]]-[[spsK]]-[[spsL]]'' {{PubMed\|9353933}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsA_3892351_3893121_-1 spsA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigK]] {{PubMed\|15383836}}
−
−	* '''Regulation:'''
−	** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed\|25239894,15383836}}
−
−	* '''Regulatory mechanism:'''
−	** [[GerE]]: transcription activation {{PubMed\|25239894,15383836}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>9353933 15383836 11733986 10350455 25239894 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 11:19, 23 September 2014
Line 51:		Line 51:

	===Phenotypes of a mutant ===		===Phenotypes of a mutant ===
		+	* reduced [[germination]] efficiency {{PubMed\|25239894}}

	=== Database entries ===		=== Database entries ===

@@ Line 41: / Line 41: @@
 = This gene is a member of the following [[regulons]] =
 {{SubtiWiki regulon|[[SigK regulon]]}}
@@ Line 106: / Line 107: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsA_3892351_3893121_-1 spsA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigK]] {{PubMed|15383836}}
+* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15383836}}
 * '''Regulation:'''
-** expressed late during sporulation in the mother cell ([[SigK]]) {{PubMed|15383836}}
+** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|25239894,15383836}}
 * '''Regulatory mechanism:'''
 * '''Additional information:'''

@@ Line 134: / Line 134: @@
 =References=
-<pubmed>9353933 15383836 11733986 10350455 </pubmed>
+<pubmed>9353933 15383836 11733986 10350455 25239894 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 66: / Line 66: @@
 * '''Catalyzed reaction/ biological activity:'''
-* '''Protein family:''' glycosyltransferase 2 family (according to Swiss-Prot)
+* '''Protein family:''' glycosyltransferase 2 family {{PubMed|10350455}}
 * '''Paralogous protein(s):'''

@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 63: / Line 59: @@
 === Additional information===
 =The protein=
@@ Line 96: / Line 89: @@
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37910&redirect=T BSU37910]
-* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QGS 1QGS] (complex with UDP),  [http://www.rcsb.org/pdb/explore.do?structureId=1H7Q 1H7Q]
+* '''Structure:'''
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P39621 P39621]
@@ Line 139: / Line 134: @@
 =References=
-<pubmed>9353933 15383836 </pubmed>
+<pubmed>9353933 15383836 11733986 10350455 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' spore coat polysaccharide synthesis <br/><br/>
+* '''Description:''' nucleotide-sugar-dependent glycosyltransferase, spore coat polysaccharide synthesis <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 10: / Line 10: @@
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
-|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+|style="background:#ABCDEF;" align="center"| '''Product''' || nucleotide-sugar-dependent glycosyltransferase
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || spore coat polysaccharide synthesis

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sps.html]
@@ Line 93: / Line 94: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QGS 1QGS] (complex with UDP),  [http://www.rcsb.org/pdb/explore.do?structureId=1H7Q 1H7Q]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsA_3892351_3893121_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:spsA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsA_3892351_3893121_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:spsA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37910]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsB]]'', ''[[gerQ]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37910 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37910 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:spsA_context.gif]]