SpoIVA - Revision history

Jstuelk: /* Original publications */

2014-05-11T13:12:12Z

‎Original publications

134.76.70.252 at 14:14, 17 April 2014

2014-04-17T14:14:56Z

134.76.38.147 at 14:04, 2 April 2014

2014-04-02T14:04:26Z

Jstuelk: /* References */

2014-01-04T18:16:04Z

‎References

Jstuelk: /* Extended information on the protein */

2014-01-04T18:13:34Z

‎Extended information on the protein

Jstuelk at 18:12, 4 January 2014

2014-01-04T18:12:45Z

Jstuelk: /* Reviews */

2014-01-04T18:10:15Z

‎Reviews

134.76.70.252 at 08:43, 12 November 2013

2013-11-12T08:43:53Z

Jstuelk: /* References */

2013-08-13T17:57:46Z

‎References

Jstuelk at 17:46, 13 August 2013

2013-08-13T17:46:07Z

← Older revision		Revision as of 13:12, 11 May 2014
Line 1:		Line 1:
−	* '''Description:~~''' ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via [[SpoVM]] <br/><br/>~~	+	{{#bank: SpoIVA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''spoIVA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''spoVP ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| ATPase, basement layer protein for spore coat assembly~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| spore cortex formation and coat assembly~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22800 spoIVA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SpoIVA SpoIVA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 55 kDa, 4.546~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1476 bp, 492 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[hbs]]'', ''[[yphF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22800 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:spoIVA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIVA_2386195_2387673_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:spoIVA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22800]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SpoIIID regulon]]}}~~
−
−	~~= SpoIVA-dependent proteins of the spore coat basement =~~
−	* [[CotE]], [[LipC]], [[SpoVM]], [[SpoVID]], [[YhaX]], [[YheD]], [[YjzB]], [[YppG]] {{PubMed\|22171814}}
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU22800
−
−	~~===Phenotypes of a mutant ===~~
−	* the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm {{PubMed\|22171814}}
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22800&redirect=T BSU22800]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIVA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10275]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** uses ATP hydrolysis to drive self-assembly into static filaments {{PubMed\|23267091}}
−	** ATP hydrolysis drives polymerization of a nucleotide-free filament {{PubMed\|23267091}}
−	** ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore {{PubMed\|23267091}}
−
−	* '''Protein family:'''
−	** belongs to the TRAFAC class of P-loop GTPases, but has lost the ability to bind GTP {{PubMed\|23267091}}
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:''' contains a Walker A ATPase domain
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[SpoIVA]]-[[SpoVID]] {{PubMed\|19702880,22262582}}
−	** [[SpoIVA]]-[[SafA]] {{PubMed\|19702880,22262582}}
−	** [[SpoIVA]]-[[SpoVM]]-[[SpoVID]] {{PubMed\|19775244}}
−
−	* '''[[Localization]]:'''
−	** innermost protein of the spore coat basement {{PubMed\|22171814}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22800&redirect=T BSU22800]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P35149 P35149]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22800]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''spoIVA'' {{PubMed\|1729246}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIVA_2386195_2387673_-1 spoIVA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigE]] {{PubMed\|1729246,15699190}}
−
−	* '''Regulation:'''
−	** expressed early during sporulation in the mother cell ([[SigE]], [[SpoIIID]]) {{PubMed\|1729246,15699190,15383836}}
−
−	* '''Regulatory mechanism:'''
−	** [[SpoIIID]]: transcription repression {{PubMed\|15383836}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 276 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 10 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 309 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed>22192522 23202530</pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>8748030, 15699190 1729246,8936302, 1729247,9922240,7592342, 8299942,1691789, 12644503, 18691972, 17427285, 19775244 22171814 15383836 23267091 19702880,22262582</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 129: / Line 129: @@
 * '''Additional information:'''
 =Biological materials =
 * '''Mutant:'''

@@ Line 58: / Line 58: @@
 * the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm {{PubMed|22171814}}
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIVA.html]
@@ Line 101: / Line 102: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 149: / Line 149: @@
 == Reviews ==
 <pubmed>22192522 23202530</pubmed>
 == Original publications ==
 <pubmed>8748030, 15699190 1729246,8936302, 1729247,9922240,7592342, 8299942,1691789, 12644503, 18691972, 17427285, 19775244 22171814 15383836 23267091 19702880,22262582</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 98: / Line 98: @@
 * '''[[Localization]]:'''
-** spore coat (basement) {{PubMed|22171814}}
+** innermost protein of the spore coat basement {{PubMed|22171814}}
 === Database entries ===

@@ Line 84: / Line 84: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:''' contains a Walker A ATPase domain
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 118: / Line 118: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIVA_2386195_2387673_-1 spoIVA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigE]] {{PubMed|1729246,15699190}}
+* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|1729246,15699190}}
 * '''Regulation:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22800 spoIVA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIVA SpoIVA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SpoIVA SpoIVA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 4.546

@@ Line 147: / Line 147: @@
 =References=
-'''Additional publications:''' {{PubMed|19702880,22262582}}
+== Reviews ==
-<pubmed>8748030, 15699190 1729246,8936302, 1729247,9922240,7592342, 8299942,1691789, 12644503, 18691972, 17427285, 19775244 22171814 15383836 23267091 </pubmed>
+<pubmed> 22192522 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 45: / Line 45: @@
 {{SubtiWiki regulon|[[SigE regulon]]}},
 {{SubtiWiki regulon|[[SpoIIID regulon]]}}
 =The gene=