Sfp/2 - Revision history

134.76.38.147 at 12:58, 2 April 2014

2014-04-02T12:58:29Z

134.76.70.252 at 12:32, 16 May 2013

2013-05-16T12:32:21Z

134.76.70.252 at 12:07, 13 May 2013

2013-05-13T12:07:58Z

134.76.70.252 at 13:20, 8 August 2012

2012-08-08T13:20:07Z

Raphael2215 at 11:24, 8 August 2012

2012-08-08T11:24:57Z

134.76.70.252 at 09:41, 8 August 2012

2012-08-08T09:41:56Z

134.76.70.252 at 08:51, 8 August 2012

2012-08-08T08:51:10Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by Raphael2215

2012-08-07T11:17:59Z

Reverted edits by 134.76.70.252 (talk) to last revision by Raphael2215

134.76.70.252 at 15:01, 6 August 2012

2012-08-06T15:01:55Z

Raphael2215 at 11:24, 19 April 2012

2012-04-19T11:24:24Z

← Older revision		Revision as of 12:58, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' 4'-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168<br~~/~~><br/>~~	+	{{#bank: Sfp/2}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''sfp/2''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''sfp''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| 4'-phosphopantetheinyl transferase~~
−	\|-
−	\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| phosphopantetheinylates a serine residue in each<br/> of the seven peptidyl carrier protein domains of the <br/>first three subunits of surfactin synthetase ([[SrfAA]]-[[SrfAB]]-[[SrfAC]]) and [[AcpK]]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03570 sfp/2]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| - , -~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 495 bp, -~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[sfp/1]]'', ''[[yczE]]''~~
−	\|-
−	~~\|style="background:#FAF8CC;" align="center"\|'''[http://subtiwiki.uni-goettingen.de/sfp_nucleotide.txt Gene sequence (+200bp) ]'''~~
−	~~\|style="background:#FAF8CC;" align="center"\|'''[http://subtiwiki.uni-goettingen.de/sfp_protein.txt Protein sequence]'''~~
−	\|-
−	~~\|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" \| '''Caution: The sequence for this gene in SubtiList contains errors~~
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:sfp_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sfp_407638_408135_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:sfp/2_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03570]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−
−	~~<br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[miscellaneous metabolic pathways]]}},~~
−	~~{{SubtiWiki category\|[[biofilm formation]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis of antibacterial compounds]]}},~~
−	~~{{SubtiWiki category\|[[pseudogenes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU03570
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~No swarming motility on B medium. [http://www.ncbi.nlm.nih.gov/sites/entrez/19202088 PubMed]~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10176]
−
−	~~=== Additional information===~~
−
−	* This gene is a pseudogene in ''B. subtilis'' 168 due to a mutation that resulted in an internal frameshift. The second part of the pseudogene is ''[[sfp/2]]''.
−	* Correction of ''[[sfp]]'', ''[[epsC]]'','' [[swrAA]]'', and ''[[degQ]]'' as well as introduction of ''rapP'' from a plasmid present in NCIB3610 results in biofilm formation in ''B. subtilis'' 168 {{PubMed\|21278284}}
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' CoA + apo-[peptidyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] (according to Swiss-Prot)
−
−	* '''Protein family:''' Gsp/sfp/hetI/acpT family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QR0 1QR0] (complex with CoA)
−
−	* '''Swiss prot entry:'''
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03570]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sfp_407638_408135_-1 sfp/2] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>20735481 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>15955059,19202088,2848009,11489886,15065855,10581256,15066026,11867633,19202088, 19749039 20094656 9484229 20148996 21278284 21466506 </pubmed>~~
−
−	~~[[Category:Pseudogenes]]~~

@@ Line 30: / Line 30: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sfp_407638_408135_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sfp/2_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sfp_407638_408135_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sfp/2_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03570]]
 |-
 |}

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || phosphopantetheinylates a serine residue in each<br/> of the seven peptidyl carrier protein domains of the <br/>first three subunits of surfactin synthetase ([[SrfAA]]-[[SrfAB]]-[[SrfAC]]) and [[AcpK]]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03570 sfp/2]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03570 sfp/2]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || - , -

@@ Line 29: / Line 29: @@
 |colspan="2" | '''Genetic context''' <br/> [[Image:sfp_context.gif]]
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
 |}
 __TOC__
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>

@@ Line 29: / Line 29: @@
 |colspan="2" | '''Genetic context''' <br/> [[Image:sfp_context.gif]]
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
 |}

@@ Line 1: / Line 1: @@
+* '''Description:''' 4'-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || phosphopantetheinylates a serine residue in each<br/> of the seven peptidyl carrier protein domains of the <br/>first three subunits of surfactin synthetase ([[SrfAA]]-[[SrfAB]]-[[SrfAC]]) and [[AcpK]]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || - , -