RecO - Revision history

Jstuelk: /* Original publications */

2015-08-10T11:07:15Z

‎Original publications

Jstuelk: /* Original publications */

2015-08-10T11:02:47Z

‎Original publications

Jstuelk at 08:02, 7 July 2015

2015-07-07T08:02:58Z

Jstuelk at 09:15, 7 May 2015

2015-05-07T09:15:26Z

Jstuelk at 11:17, 4 June 2014

2014-06-04T11:17:19Z

134.76.38.147 at 14:15, 2 April 2014

2014-04-02T14:15:08Z

Jstuelk at 17:22, 29 November 2013

2013-11-29T17:22:34Z

Fabian Commichau: /* Original publications */

2013-11-29T11:37:10Z

‎Original publications

134.76.70.252 at 08:53, 12 November 2013

2013-11-12T08:53:32Z

Jstuelk at 16:13, 13 September 2013

2013-09-13T16:13:55Z

← Older revision		Revision as of 11:07, 10 August 2015
Line 1:		Line 1:
−	* '''Description:''' mediator of [[RecA]] binding to ssDNA, required for the formation of [[RecA]] DNA repair centers, , required for efficient survival and replication restart after replication-transcription conflicts <br/><br/>	+	{{#bank: RecO}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''recO''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yqxN, yqfI ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| mediator of [[RecA]] binding to ssDNA~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[DNA repair/ recombination]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280 recO]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecO RecO]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 29 kDa, 8.208~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 765 bp, 255 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[glyQ]]'', ''[[yqzL]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25280 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:recO_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recO_2608946_2609713_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:recO_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA repair/ recombination]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU25280
−
−	~~===Phenotypes of a mutant ===~~
−	* drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) {{PubMed\|24285298}}
−	* ''[[recO]] [[recJ]]'' double mutants are extremely sensitive against DNA damaging agents {{PubMed\|26001966}}
−	* ''[[recO]] [[addA]]-[[addB]]'' double mutants are extremely sensitive against DNA damaging agents {{PubMed\|26001966}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25280&redirect=T BSU25280]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11189]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[DprA]]) {{PubMed\|22373918}}
−	** catalyzes annealing of [[SsbA]] or [[SsbA]]/[[SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation {{PubMed\|22373918}}
−	** required for the formation of [[RecA]] DNA repair centers (together with [[RecR]]) {{PubMed\|24891441}}
−
−	* '''Protein family:''' recO family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[SsbA]] (bound to ssDNA) -[[RecO]], this helps to dislodge [[SsbA]] and [[SsbB]] from ssDNA {{PubMed\|21170359,22373918}}
−	** [[RecR]]-[[RecO]], [[RecR]]:[[RecO]] molecular ratio of 2:1 (as judged from other bacteria such as ''E. coli'' and ''Deinococcus radiodurans'') {{PubMed\|17581636}}
−
−	* '''[[Localization]]:'''
−	** cytoplasm (according to Swiss-Prot)
−	** localizes to the DNA entry pole during transformation {{PubMed\|22373918}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25280&redirect=T BSU25280]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2V1C 2V1C] (the [[RecR]]-[[RecO]] complex from ''Deinococcus radiodurans'', [[RecR]]: 52% identity, 78% similarity, [[RecO]]: 30%/ 58%) {{PubMed\|17581636}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P42095 P42095]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25280]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recO_2608946_2609713_-1 recO] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A892 ( ''recO''::''cat''), {{PubMed\|10323239}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 22933559 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832 26001966 15186413</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 151: / Line 151: @@
 <pubmed> 22933559 </pubmed>
 == Original publications ==
-<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832 26001966</pubmed>
+<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832 26001966 15186413</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
 * drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) {{PubMed|24285298}}
 === Database entries ===
@@ Line 149: / Line 151: @@
 <pubmed> 22933559 </pubmed>
 == Original publications ==
-<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832</pubmed>
+<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832 26001966</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' mediator of [[RecA]] binding to ssDNA, required for the formation of [[RecA]] DNA repair centers <br/><br/>
+* '''Description:''' mediator of [[RecA]] binding to ssDNA, required for the formation of [[RecA]] DNA repair centers, , required for efficient survival and replication restart after replication-transcription conflicts <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 149: / Line 149: @@
 <pubmed> 22933559 </pubmed>
 == Original publications ==
-<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298</pubmed>
+<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' mediator of [[RecA]] binding to ssDNA <br/><br/>
+* '''Description:''' mediator of [[RecA]] binding to ssDNA, required for the formation of [[RecA]] DNA repair centers <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 72: / Line 72: @@
 ** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[DprA]]) {{PubMed|22373918}}
 ** catalyzes annealing of [[SsbA]] or [[SsbA]]/[[SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation {{PubMed|22373918}}
 * '''Protein family:''' recO family (according to Swiss-Prot)
@@ Line 148: / Line 149: @@
 <pubmed> 22933559 </pubmed>
 == Original publications ==
-<pubmed>,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298</pubmed>
+<pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 57: / Line 57: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 97: / Line 98: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280 recO]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RecO RecO]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecO RecO]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 8.208

@@ Line 39: / Line 39: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 146: / Line 142: @@
 =References=
-'''Additional publications:''' {{PubMed|21170359}}
+== Reviews ==
-<pubmed>,18599486, 19730681 20581116 22373918 17581636</pubmed>
+<pubmed> 22933559 </pubmed>
 [[Category:Protein-coding genes]]