QueF - Revision history

134.76.70.252 at 09:43, 17 April 2014

2014-04-17T09:43:47Z

2014-04-02T13:33:22Z

2013-05-16T13:07:23Z

2013-05-14T10:03:20Z

2013-05-13T12:40:35Z

2012-09-06T08:28:05Z

2012-08-07T08:57:10Z

2012-07-13T09:01:46Z

2012-04-19T08:17:28Z

2012-04-13T07:46:14Z

← Older revision		Revision as of 09:43, 17 April 2014
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	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 303 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 913 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

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 === Database entries ===
 * '''DBTBS entry:''' no entry
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 === Database entries ===
 * '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=4F8b 4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [[QueF]]) {{PubMed|22787148}}

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   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queF_1441291_1441788_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:queF_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queF_1441291_1441788_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:queF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13750]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[queE]]'', ''[[ykvN]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13750 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13750 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:ykvM_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13750 queF]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13750 queF]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.927
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[queE]]'', ''[[ykvN]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13248&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13750 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:ykvM_context.gif]]

@@ Line 82: / Line 82: @@
 * '''Domains:'''
-* '''Modification:'''
+* '''Modification:''' active site Cys56 is S-bacillithiolated by NaOCl stress {{PubMed|22938038}}
 * '''Cofactor(s):'''
@@ Line 140: / Line 140: @@
 =References=
-<pubmed>14660578,15767583 16511203 ,17929836 19285444 17384645 22787148 </pubmed>
+<pubmed>22938038,14660578,15767583 16511203 ,17929836 19285444 17384645 22787148 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' nitrile reductase, synthesis of the modified ribonucleotide queuosine <br/><br/>
+* '''Description:''' nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 92: / Line 92: @@
 === Database entries ===
-* '''Structure:'''
+* '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=4F8b 4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [[QueF]]) {{PubMed|22787148}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/O31678 O31678]
@@ Line 138: / Line 138: @@
 =References=
-<pubmed>14660578,15767583 16511203 ,17929836 19285444 17384645 </pubmed>
+<pubmed>14660578,15767583 16511203 ,17929836 19285444 17384645 22787148 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 98: / Line 98: @@
 * '''Operon:''' ''[[queC]]-[[queD]]-[[queE]]-[[queF]]'' {{PubMed|14660578}}
-* '''[[Sigma factor]]:'''
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queF_1441291_1441788_1 queF] {{PubMed|22383849}}
 * '''Regulation:'''
@@ Line 106: / Line 108: @@
 ** [[preQ1 riboswitch]]: transcriptional antitermination in the absence of queuosine {{PubMed|19285444}}
 * '''Additional information:'''
 =Biological materials =