Pgk - Revision history

Jstuelk at 17:15, 16 May 2014

2014-05-16T17:15:54Z

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Jstuelk at 17:14, 16 May 2014

2014-05-16T17:14:30Z

134.76.70.252 at 14:11, 17 April 2014

2014-04-17T14:11:42Z

134.76.70.252 at 09:26, 17 April 2014

2014-04-17T09:26:39Z

134.76.38.147 at 14:47, 2 April 2014

2014-04-02T14:47:33Z

Jstuelk at 14:37, 5 March 2014

2014-03-05T14:37:28Z

134.76.70.252 at 11:41, 7 January 2014

2014-01-07T11:41:39Z

Jstuelk at 15:36, 13 July 2013

2013-07-13T15:36:16Z

134.76.70.252 at 14:36, 16 May 2013

2013-05-16T14:36:06Z

134.76.70.252 at 11:17, 14 May 2013

2013-05-14T11:17:58Z

@@ Line 57: / Line 57: @@
 ===Phenotypes of a mutant ===
 * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}}
 === Database entries ===
@@ Line 161: / Line 162: @@
 =References=
-<pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519 15378759</pubmed>
+<pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519 15378759 24825009</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 132: / Line 132: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 12006 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 10462 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''
 ** GP699 (''pgk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}lab

@@ Line 130: / Line 130: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1PHP 1PHP] (from ''Geobacillus stearothermophilus'')

@@ Line 43: / Line 43: @@
 {{SubtiWiki category|[[carbon core metabolism]]}},
 {{SubtiWiki category|[[phosphoproteins]]}},
-{{SubtiWiki category|[[universally conserved proteins]]}}
+{{SubtiWiki category|[[universally conserved proteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 64: / Line 65: @@
 === Additional information===
 =The protein=
@@ Line 80: / Line 79: @@
 * '''Kinetic information:''' Two Substrate Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
-* '''Domains:'''
+* '''[[Domains]]:'''
 ** nucleotide binding domain (ATP) (350–353)
 ** 2x substrate binding domain (21–23), (59–62)
@@ Line 86: / Line 85: @@
 * '''Modification:''' phosphorylation on Ser-183 AND Thr-299 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
-* '''Cofactor(s):''' Mg2+ or Mn2+ [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
+* '''[[Cofactors]]:''' Mg2+ or Mn2+ [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed]
 * '''Effectors of protein activity:'''
@@ Line 128: / Line 127: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 155: / Line 155: @@
 =References=
-<pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519</pubmed>
+<pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33930 pgk]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pgk pgk]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42,0 kDa, 4.77

@@ Line 132: / Line 132: @@
 * '''Mutant:'''
-** GP699 (''pgk''::''cat''), available in [[Stülke]] lab
+** GP699 (''pgk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}lab
-** GP707 (''pgk''::''erm''), available in [[Stülke]] lab
+** GP707 (''pgk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
 * '''Expression vector:'''
-** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
+** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
-** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Stülke]] lab
+** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
-** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab
+** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Jörg Stülke]]'s lab
-** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
+** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
-* '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Stülke]] lab
+* '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available iin [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgk_3480197_3481381_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pgk_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgk_3480197_3481381_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pgk_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33930]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tpi]]'', ''[[gapA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33930 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33930 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:pgk_context.gif]]