PHT43 - Revision history

Bzhu at 11:34, 28 August 2017

2017-08-28T11:34:50Z

Bzhu at 09:23, 28 August 2017

2017-08-28T09:23:51Z

Bzhu at 13:13, 21 August 2017

2017-08-21T13:13:16Z

Bzhu: Created page with "{{ContentDisclamer}} 600px 230px The pHT43 is a high-level expression vector for recombinant protein production and secretion into the..."

2017-08-21T13:09:07Z

Created page with "{{ContentDisclamer}} 600px 230px The pHT43 is a high-level expression vector for recombinant protein production and secretion into the..."

New page

{{ContentDisclamer}}

[[File:PHT43.PNG|600px]]
[[File:Legend.png|230px]]

The pHT43 is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.

=== Features ===

* Secretory protein production
* Efficiently controllable gene expression (IPTG inducible)
* High level production and secretion of recombinant proteins
* ''E. coli / B. subtilis'' shuttle vector

pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N, for secretory protein production are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]

== References ==
<pubmed>17624574, 16005967, 16125412</pubmed>

@@ Line 2: / Line 2: @@
 [[File:PHT43.PNG|600px]]
-[[File:Legend.png|230px]]
+[[File:PHT43-Legende.jpg|300px]]
 The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.

@@ Line 13: / Line 13: @@
 * ''E. coli / B. subtilis'' shuttle vector
-pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N,  for secretory protein production  are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]
+pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N,  for secretory protein production  are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]]
 == References ==
 <pubmed>17624574, 16005967, 16125412</pubmed>

@@ Line 4: / Line 4: @@
 [[File:Legend.png|230px]]
-The pHT43 is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.
+The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.
 === Features ===