https://subtiwiki.uni-goettingen.de/wiki//index.php?action=history&feed=atom&title=PBP43 2026-04-05T00:25:59Z Revision history for this page on the wiki MediaWiki 1.30.0 https://subtiwiki.uni-goettingen.de/wiki//index.php?title=PBP43&diff=209823&oldid=prev 2017-11-22T13:18:29Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr style="vertical-align: top;" lang="en"> <td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 13:18, 22 November 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13" >Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* Don't use XbaI and KpnI for cloning. They cut the vector twice!</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* Don't use XbaI and KpnI for cloning. They cut the vector twice!</div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">'''The reference'''</ins></div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">&lt;pubmed&gt;&#160; 27708634&lt;/pubmed&gt;</ins></div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>==&#160; Return to [[Plasmids]] ==</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>==&#160; Return to [[Plasmids]] ==</div></td></tr> </table>

Jstuelk https://subtiwiki.uni-goettingen.de/wiki//index.php?title=PBP43&diff=192292&oldid=prev 2013-06-03T13:54:04Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr style="vertical-align: top;" lang="en"> <td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 13:54, 3 June 2013</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13" >Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* Don't use XbaI and KpnI for cloning. They cut the vector twice!</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* Don't use XbaI and KpnI for cloning. They cut the vector twice!</div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">==&#160; Return to [[Plasmids]] ==</ins></div></td></tr> </table>

Jstuelk https://subtiwiki.uni-goettingen.de/wiki//index.php?title=PBP43&diff=178898&oldid=prev 2013-03-01T12:12:44Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr style="vertical-align: top;" lang="en"> <td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 12:12, 1 March 2013</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l12" >Line 12:</td> <td colspan="2" class="diff-lineno">Line 12:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* take care that the integration is in-frame</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* take care that the integration is in-frame</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Don't use XbaI and KpnI for cloning. They cut the vector twice!</ins></div></td></tr> </table>

Kgunka https://subtiwiki.uni-goettingen.de/wiki//index.php?title=PBP43&diff=178897&oldid=prev 2013-03-01T12:11:26Z

<p>Created page with &quot;<a href="/wiki//index.php?title=File:PBP43.png" title="File:PBP43.png">250px|thumb|right|&#039;&#039;&#039;pBP43: click to enlarge&#039;&#039;&#039;</a> &#039;&#039;&#039;General comments&#039;&#039;&#039; Integrative <a href="/wiki//index.php?title=Plasmid" class="mw-redirect" title="Plasmid">plasmid</a> for the fusion of the monomeric variant of GFP A206K to the C-...&quot;</p> <p><b>New page</b></p><div>[[File:pBP43.png|250px|thumb|right|'''pBP43: click to enlarge''']]<br /> <br /> '''General comments'''<br /> <br /> Integrative [[plasmid]] for the fusion of the monomeric variant of GFP A206K to the C-terminus of a protein, keeping the control of the expression under its natural promoter<br /> * ''E.coli'': multicopy plasmid, select for ampicillin<br /> * ''B. subtilis'': no ori, select for spectinomycin<br /> <br /> '''Hints for the usage'''<br /> * amplify the last 600bp of your gene of interest, <br /> * skip the Stopp-Codon of the gene in the reverse primer<br /> * take care that the integration is in-frame<br /> * do not use the HindIII site for cloning, as GFP is inserted via this restriction site</div>

Kgunka