MurG - Revision history

Jstuelk: /* References */

2015-08-19T15:05:42Z

‎References

Jstuelk at 13:02, 8 May 2015

2015-05-08T13:02:24Z

Jgundlach at 15:53, 15 August 2014

2014-08-15T15:53:38Z

134.76.70.252 at 09:54, 17 April 2014

2014-04-17T09:54:35Z

Jstuelk at 15:21, 8 April 2014

2014-04-08T15:21:23Z

134.76.38.147 at 13:39, 2 April 2014

2014-04-02T13:39:07Z

Jstuelk at 09:22, 5 March 2014

2014-03-05T09:22:51Z

Jstuelk at 09:22, 5 March 2014

2014-03-05T09:22:28Z

134.76.70.252 at 10:43, 7 January 2014

2014-01-07T10:43:17Z

134.76.70.252 at 13:14, 16 May 2013

2013-05-16T13:14:40Z

← Older revision		Revision as of 15:05, 19 August 2015
Line 1:		Line 1:
−	* '''Description:~~''' UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II <br/><br/>~~	+	{{#bank: MurG}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''murG''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| UDP-N-acetylglucosamine-~~
−	~~N-acetylmuramyl-(pentapeptide)pyrophosphoryl-<br/>~~
−	~~undecaprenol N-acetylglucosamine transferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| peptidoglycan precursor biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15220 murG]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=murG murG]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 39 kDa, 9.568~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1089 bp, 363 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[spoVE]]'', ''[[murB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15220 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15220 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15220 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:murG_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murG_1591540_1592631_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:murG_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15220]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall synthesis]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis of cell wall components]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SpoIIID regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU15220
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15220&redirect=T BSU15220]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10227]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule
−	** UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)
−
−	* '''Protein family:''' MurG subfamily (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** peripheral membrane protein {{PubMed\|24706874}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15220&redirect=T BSU15220]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1F0K 1F0K] (from ''Escherichia coli'', 30% identity, 52% similarity) {{PubMed\|10892798}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37585 P37585]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15220]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.4.1.227 2.4.1.227]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−	** ''[[murE]]-[[mraY]]-[[murD]]-[[spoVE]]-[[murG]]-[[murB]]-[[divIB]]-[[ylxW]]-[[ylxX]]-[[sbp]]'' {{PubMed\|1391053}}
−	** ''[[spoVE]]-[[murG]]-[[murB]]-[[divIB]]-[[ylxW]]-[[ylxX]]-[[sbp]]'' {{PubMed\|1391053}}
−	** ''[[murG]]-[[murB]]-[[divIB]]-[[ylxW]]-[[ylxX]]-[[sbp]]'' {{PubMed\|18179421}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murG_1591540_1592631_1 murG] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−	** ''[[spoVE]]'': [[SigE]] {{PubMed\|8320223}}
−	** within ''[[murG]]'': [[SigM]] {{PubMed\|18179421}}
−
−	* '''Regulation:'''
−	** expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([[SigE]]) {{PubMed\|1391053}}
−
−	* '''Regulatory mechanism:'''
−	** [[SpoIIID]]: transcription repression {{PubMed\|15383836}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 82 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 627 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''FLAG-tag construct:'''
−	** GP2002 ''murG-3xFLAG ermC'' (based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed> 22092867,18063720,18081839, 1391053 8320223 15383836 18179421 10790316 24706874 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 13:02, 8 May 2015
Line 1:		Line 1:
−	* '''Description:''' UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase <br/><br/>	+	* '''Description:''' UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II <br/><br/>

	{\| align="right" border="1" cellpadding="2"		{\| align="right" border="1" cellpadding="2"

← Older revision		Revision as of 15:53, 15 August 2014
Line 149:		Line 149:

	* '''two-hybrid system:'''		* '''two-hybrid system:'''
		+
		+	* '''FLAG-tag construct:'''
		+	** GP2002 ''murG-3xFLAG ermC'' (based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab

	* '''Antibody:'''		* '''Antibody:'''

← Older revision		Revision as of 09:54, 17 April 2014
Line 135:		Line 135:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 82 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 627 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 77: / Line 77: @@
 === Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:''' UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)
+* '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' MurG subfamily (according to Swiss-Prot)
@@ Line 97: / Line 99: @@
 * '''[[SubtInteract|Interactions]]:'''
-* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
+* '''[[Localization]]:'''
 === Database entries ===
@@ Line 121: / Line 124: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murG_1591540_1592631_1 murG] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 ** ''[[spoVE]]'': [[SigE]] {{PubMed|8320223}}
 ** within ''[[murG]]'': [[SigM]] {{PubMed|18179421}}
@@ Line 153: / Line 156: @@
 =References=
-<pubmed> 22092867,18063720,18081839, 1391053 8320223 15383836 18179421 10790316 </pubmed>
+<pubmed> 22092867,18063720,18081839, 1391053 8320223 15383836 18179421 10790316 24706874 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 65: / Line 65: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp.html]
@@ Line 99: / Line 100: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1F0K 1F0K] (from ''Escherichia coli'', 30% identity, 52% similarity) {{PubMed|10892798}}

@@ Line 11: / Line 11: @@
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || UDP-N-acetylglucosamine-
-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-
+N-acetylmuramyl-(pentapeptide)pyrophosphoryl-<br/>
 undecaprenol N-acetylglucosamine transferase
 |-

@@ Line 11: / Line 11: @@
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || UDP-N-acetylglucosamine-
-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
+N-acetylmuramyl-(pentapeptide)pyrophosphoryl-
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis
@@ Line 40: / Line 41: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 74: / Line 71: @@
 === Additional information===
 =The protein=
@@ Line 92: / Line 86: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''

@@ Line 17: / Line 17: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15220 murG]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=murG murG]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 9.568

@@ Line 32: / Line 32: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murG_1591540_1592631_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:murG_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murG_1591540_1592631_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:murG_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15220]]
 |-
 |}