MleA - Revision history

134.76.70.252 at 09:55, 17 April 2014

2014-04-17T09:55:10Z

134.76.38.147 at 14:08, 2 April 2014

2014-04-02T14:08:18Z

134.76.70.252 at 11:08, 7 January 2014

2014-01-07T11:08:46Z

134.76.70.252 at 13:50, 16 May 2013

2013-05-16T13:50:05Z

134.76.70.252 at 10:38, 14 May 2013

2013-05-14T10:38:37Z

134.76.70.252 at 13:13, 13 May 2013

2013-05-13T13:13:08Z

Jstuelk: /* References */

2012-11-10T11:07:41Z

‎References

Jstuelk: /* References */

2012-11-10T11:07:09Z

‎References

Jstuelk at 16:13, 23 August 2012

2012-08-23T16:13:00Z

Jstuelk at 16:07, 23 August 2012

2012-08-23T16:07:54Z

← Older revision		Revision as of 09:55, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' malic enzyme<br/><br/>~~	+	{{#bank: MleA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mleA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yqkJ ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| NAD-dependent malate dehydrogenase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| malate utilization~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23550 mleA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mleA mleA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 45 kDa, 4.895~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1317 bp, 439 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yqkK]]'', ''[[mleN]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23550 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mleA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mleA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23550]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[AnsR regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU23550
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23550&redirect=T BSU23550]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11764]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' (S)-malate + NAD<sup>+</sup> = pyruvate + CO<sub>2</sub> + NADH (according to Swiss-Prot) malate --> pyruvate
−
−	* '''Protein family:''' malic enzymes family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[YtsJ]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23550&redirect=T BSU23550]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54572 P54572]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23550]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.38 1.1.1.38]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−	** ''[[ansA]]-[[ansB]]-[[mleN]]-[[mleA]]'' {{PubMed\|22383849}}
−	** ''[[mleN]]-[[mleA]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 mleA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−	** ''[[ansA]]'': [[SigA]] {{PubMed\|1711029}}
−	** ''[[mleN]]'': [[SigA]] {{PubMed\|22900538}}
−
−	* '''Regulation:'''
−	** ''[[ansA]]'': expressed in the presence of asparagine ([[AnsR]]) {{PubMed\|11914346}}
−	** ''[[mleN]]'': repressed by glucose ([[CcpA]]) {{PubMed\|22900538}}
−
−	* '''Regulatory mechanism:'''
−	** [[AnsR]]: transcription repression {{PubMed\|11914346}}
−	** [[CcpA]]: transcription repression {{PubMed\|22900538}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' GP1136 (cat) available in [[Stülke]] lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~'''Additional publications:''' {{PubMed\|22900538}}~~
−	~~<pubmed> 16788182 11914346 22383849 23136871</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 90: / Line 91: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23550 mleA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mleA mleA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.895

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mleA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mleA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23550]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqkK]]'', ''[[mleN]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23550 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23550 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mleA_context.gif]]

@@ Line 142: / Line 142: @@
 =References=
-'''Additional publications:''' {{PubMed|22383849}}
+'''Additional publications:''' {{PubMed|22900538}}
-<pubmed> 16788182 22900538 11914346 23136871</pubmed>
+<pubmed> 16788182 11914346 22383849 23136871</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 110: / Line 110: @@
 * '''Sigma factor:'''
 ** ''[[mleN]]'': [[SigA]] {{PubMed|22900538}}
 * '''Regulation:'''
-** repressed by glucose ([[CcpA]]) {{PubMed|22900538}}
+** ''[[ansA]]'': expressed in the presence of asparagine ([[AnsR]]) {{PubMed|11914346}}
 * '''Regulatory mechanism:'''
 ** [[CcpA]]: transcription repression {{PubMed|22900538}}
@@ Line 139: / Line 142: @@
 =References=
-<pubmed> 16788182 22383849 22900538</pubmed>
+<pubmed> 16788182 22383849 22900538 11914346</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/><br/><br/><br/><br/>
@@ Line 47: / Line 43: @@
 = This gene is a member of the following [[regulons]] =
+{{SubtiWiki regulon|[[AnsR regulon]]}},
 =The gene=
@@ Line 64: / Line 61: @@
 === Additional information===
@@ Line 109: / Line 104: @@
 * '''Operon:'''
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 mleA] {{PubMed|22383849}}
 * '''Sigma factor:'''
 * '''Regulation:'''
 * '''Regulatory mechanism:'''
 * '''Additional information:'''
@@ Line 139: / Line 139: @@
 =References=
-<pubmed> 16788182 </pubmed>
+<pubmed> 16788182 22383849 22900538</pubmed>
 [[Category:Protein-coding genes]]