MinC - Revision history

Dtoedter: /* Biological materials */

2015-03-06T10:44:17Z

‎Biological materials

Jstuelk at 16:00, 27 November 2014

2014-11-27T16:00:49Z

Jstuelk: /* Biological materials */

2014-05-20T13:35:47Z

‎Biological materials

134.76.70.252 at 10:00, 17 April 2014

2014-04-17T10:00:11Z

134.76.38.147 at 14:24, 2 April 2014

2014-04-02T14:24:11Z

Jstuelk at 14:06, 27 December 2013

2013-12-27T14:06:32Z

Jstuelk at 18:07, 21 December 2013

2013-12-21T18:07:26Z

134.76.70.252 at 09:01, 12 November 2013

2013-11-12T09:01:55Z

Jstuelk at 16:59, 27 May 2013

2013-05-27T16:59:30Z

134.76.70.252 at 14:09, 16 May 2013

2013-05-16T14:09:54Z

← Older revision		Revision as of 10:44, 6 March 2015
Line 1:		Line 1:
−	* '''Description:''' cell-division inhibitor (septum placement), destabilizes [[FtsZ]]-rings at new cell poles, part of the Min system (with [[DivIVA]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ\|Z ring]] placement <br/><br/>	+	{{#bank: MinC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''minC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| cell-division inhibitor~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| septum placement~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000 minC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MinC MinC]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 24 kDa, 6.262~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 678 bp, 226 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[minD]]'', ''[[mreD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28000 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:minC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:minC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell division]]}},~~
−	~~{{SubtiWiki category\|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigH regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigM regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU28000
−
−	~~===Phenotypes of a mutant ===~~
−	* a ''[[yvcL]] [[minC]]-[[minD]]'' mutant grows poorly, and the cells are filamentous {{PubMed\|24097947}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28000&redirect=T BSU28000]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10329]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle {{PubMed\|20352045}}
−	** binds the C-terminal domain of [[FtsZ]] to inhibit its polymerization {{PubMed\|23577149}}
−
−	* '''Protein family:''' minC family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed\|20352045}}
−	** [[FtsZ]] (C-terminal domain)-[[MinC]] {{PubMed\|23577149}}
−
−	* '''[[Localization]]:'''
−	** polar/ septal at the cell membrane {{PubMed\|20566861}}
−	** membrane binding/ polar localization depends on the proton motive force {{PubMed\|20566861}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28000&redirect=T BSU28000]
−
−	* '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=2M4I 2M4I] (N-terminal domain, 105 aa)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q01463 Q01463]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28000]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:'''
−	** ''[[radC]]-[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
−	** ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' {{PubMed\|8459776}}
−	** ''[[minC]]-[[minD]]'' {{PubMed\|8459776}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 minC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−	** ''[[radC]]'': [[SigM]] {{PubMed\|18179421}}
−	** ''[[minC]]'': [[SigH]] {{PubMed\|8459776}}
−
−	* '''Regulation:'''
−	** expression is reduced in a [[SigV]] mutant {{PubMed\|21926231}}
−	** constitutively expressed {{PubMed\|23701187}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 95 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' BSN375 (''[[minC]]''::''aphA3'') (available in [[Leendert Hamoen]]'s, [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs)
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~==Reviews==~~
−	~~<pubmed> 19680248 19884039 24367361</pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 24097947 18179421 22457634 23577149 21926231 23701187 24366721</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 101: / Line 101: @@
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28000&redirect=T BSU28000]
-* '''Structure:'''
+* '''Structure:'''	[http://pdb.org/pdb/explore/explore.do?structureId=2M4I 2M4I] (N-terminal domain, 105 aa)
 * '''UniProt:''' [http://www.uniprot.org/uniprot/Q01463 Q01463]

@@ Line 134: / Line 134: @@
 =Biological materials =
-* '''Mutant:'''
+* '''Mutant:''' BSN375 (''[[minC]]''::''aphA3'') (available in [[Leendert Hamoen]]'s, [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs)
 * '''Expression vector:'''

← Older revision		Revision as of 10:00, 17 April 2014
Line 130:		Line 130:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 95 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 150: / Line 150: @@
 ==Reviews==
-<pubmed> 19680248 19884039 </pubmed>
+<pubmed> 19680248 19884039 24367361</pubmed>
 ==Original Publications==
-<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 24097947 18179421 22457634 23577149 21926231 23701187</pubmed>
+<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 24097947 18179421 22457634 23577149 21926231 23701187 24366721</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 63: / Line 64: @@
 === Additional information===
 =The protein=
@@ Line 83: / Line 81: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 154: / Line 152: @@
 <pubmed> 19680248 19884039 </pubmed>
 ==Original Publications==
-<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 18179421 22457634 23577149 21926231 23701187</pubmed>
+<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 24097947 18179421 22457634 23577149 21926231 23701187</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000 minC]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MinC MinC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MinC MinC]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 6.262

@@ Line 125: / Line 125: @@
 * '''Regulation:'''
 ** expression is reduced in a [[SigV]] mutant {{PubMed|21926231}}
 * '''Regulatory mechanism:'''
@@ Line 153: / Line 154: @@
 <pubmed> 19680248 19884039 </pubmed>
 ==Original Publications==
-<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 18179421 22457634 23577149 21926231</pubmed>
+<pubmed>8459776,19019154,1400224,10411726,15317782, 20352045,18588879, 19141479 20566861 20711458 18179421 22457634 23577149 21926231 23701187</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:minC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minC_2858584_2859264_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:minC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28000]]
 |-
 |}