McsB - Revision history

Raphael2215: /* Biological materials */

2015-07-21T13:09:09Z

‎Biological materials

Raphael2215: /* Biological materials */

2015-07-21T13:08:51Z

‎Biological materials

Jstuelk at 09:07, 23 January 2015

2015-01-23T09:07:18Z

Jstuelk: /* Original Publications */

2014-05-18T15:07:09Z

‎Original Publications

134.76.70.252 at 09:59, 17 April 2014

2014-04-17T09:59:55Z

134.76.38.147 at 12:48, 2 April 2014

2014-04-02T12:48:03Z

134.76.70.252 at 09:59, 7 January 2014

2014-01-07T09:59:35Z

Jstuelk at 07:26, 16 December 2013

2013-12-16T07:26:33Z

134.76.70.252 at 14:59, 11 November 2013

2013-11-11T14:59:43Z

134.76.70.252 at 12:20, 16 May 2013

2013-05-16T12:20:42Z

← Older revision		Revision as of 13:09, 21 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[protein kinases and phosphatases\|protein arginine kinase]], adaptor protein, modulator of [[CtsR]]-dependent repression <br/><br/>~~	+	{{#bank: McsB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mcsB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yacI ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[protein kinases and phosphatases\|protein arginine kinase]]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of [[CtsR]] activity~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850 mcsB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=McsB McsB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mcsB mcsB]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 40 kDa, 5.068~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1089 bp, 363 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[mcsA]]'', ''[[clpC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00850 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mcsB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mcsB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein modification]]}},~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}},~~
−	~~{{SubtiWiki category\|[[general stress proteins (controlled by SigB)]]}},~~
−	~~{{SubtiWiki category\|[[heat shock proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CtsR regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigB regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigF regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU00850
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00850&redirect=T BSU00850]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10147]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' targets non-functional CtsR for degradation by [[ClpP]]/[[ClpC]] {{PubMed\|20852588}}
−
−	* '''Protein family:''' ATP:guanido phosphotransferase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** autophosphorylation on specific arginine residues {{PubMed\|20852588,21622759}}, dephosphorylation by [[YwlE]] {{PubMed\|20852588,21622759}}
−	** autophosphorylation stimulates McsB activity as adaptor protein for [[ClpC]] {{PubMed\|21622759}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[McsA]]-[[McsB]]-[[CtsR]], [[McsB]]-[[CtsR]] {{PubMed\|20852588}}, [[McsA]]-[[McsB]]
−	** [[McsB]]-[[YwlE]] {{PubMed\|20852588}}
−	** [[McsB]]-[[ClpP]]/[[ClpC]] (rapid degradation of phosphorylated McsB) {{PubMed\|20852588}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00850&redirect=T BSU00850]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37570 P37570]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00850]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ctsR]]-[[mcsA]]-[[mcsB]]-[[clpC]]-[[radA]]-[[disA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 mcsB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [[SigF]] {{PubMed\|16497325}}
−
−	* '''Regulation:'''
−	** expressed early during sporulation in the forespore ([[SigF]]) {{PubMed\|16497325}}
−	** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
−	** induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9987115 PubMed]
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 142 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** ''mcsB::aphA3'' availbale from the Gerth lab
−	** mcsBC167S::spec available from the Gerth lab
−	** GP1457 (''mcsB''::''aphA3''), available in [[Jörg Stülke]]'s lab
−	** BP69 (spc), available in [[Fabian Commichau]]'s lab
−
−	* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
−	** expression of native ''mcsB'' in ''B. subtilis'': pBP529186 (in [[pBQ200]]), available in [[Fabian Commichau]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:''' available in Gerth lab
−
−	~~=Labs working on this gene/protein=~~
−
−	* [[Ulf Gerth]], Greifswald, Germany
−
−	* [[Fabian Commichau]] Göttingen, Germany
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~==Reviews==~~
−
−	~~<pubmed> 23375660 19609260</pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382 25610436</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 13:08, 21 July 2015
Line 142:		Line 142:

	* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab		* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
		+	** expression of native ''mcsB'' in ''B. subtilis'': pBP529186 (in [[pBQ200]]), available in [[Fabian Commichau]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 136: / Line 136: @@
 * '''Mutant:'''
-**''mcsB::aphA3'' availbale from the Gerth lab
+** ''mcsB::aphA3'' availbale from the Gerth lab
-**mcsBC167S::spec available from the Gerth lab
+** mcsBC167S::spec available from the Gerth lab
-**GP1457 (''mcsB''::''aphA3''),  available in [[Stülke]] lab
+** GP1457 (''mcsB''::''aphA3''),  available in [[Jörg Stülke]]'s lab
-**BP69 (spc), available in [[Fabian Commichau]]'s lab
+** BP69 (spc), available in [[Fabian Commichau]]'s lab
 * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
@@ Line 154: / Line 155: @@
 * [[Ulf Gerth]], Greifswald, Germany
-* [[Fabian_Commichau|Fabian Commichau]] Göttingen, Germany
+* [[Fabian Commichau]] Göttingen, Germany
 =Your additional remarks=
@@ Line 164: / Line 165: @@
 <pubmed> 23375660 19609260</pubmed>
 ==Original Publications==
-<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382</pubmed>
+<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382 25610436</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 164: / Line 164: @@
 <pubmed> 23375660 19609260</pubmed>
 ==Original Publications==
-<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24263382</pubmed>
+<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382</pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 09:59, 17 April 2014
Line 131:		Line 131:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 142 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 62: / Line 62: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
@@ Line 101: / Line 102: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=McsB McsB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mcsB mcsB]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.068

@@ Line 83: / Line 83: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
@@ Line 89: / Line 89: @@
 ** autophosphorylation stimulates McsB activity as adaptor protein for [[ClpC]] {{PubMed|21622759}}
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 158: / Line 158: @@
 ==Reviews==
-'''Additional reviews:''' {{PubMed|19609260}}
-<pubmed> 23375660 </pubmed>
+<pubmed> 23375660 19609260</pubmed>
 ==Original Publications==
-<pubmed> 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742</pubmed>
+<pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24263382</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850 mcsB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/McsB McsB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=McsB McsB]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mcsB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mcsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850]]
 |-
 |}