GltB - Revision history

Jstuelk: /* References */

2015-07-30T09:16:15Z

‎References

Jstuelk: /* Biological materials */

2014-09-24T08:13:26Z

‎Biological materials

134.76.70.252 at 09:58, 17 April 2014

2014-04-17T09:58:51Z

134.76.38.147 at 13:52, 2 April 2014

2014-04-02T13:52:17Z

134.76.70.252 at 10:55, 7 January 2014

2014-01-07T10:55:03Z

134.76.70.252 at 08:33, 12 November 2013

2013-11-12T08:33:08Z

134.76.70.252 at 13:31, 16 May 2013

2013-05-16T13:31:09Z

134.76.70.252 at 10:22, 14 May 2013

2013-05-14T10:22:52Z

134.76.70.252 at 12:58, 13 May 2013

2013-05-13T12:58:18Z

Jstuelk at 14:07, 28 March 2013

2013-03-28T14:07:21Z

← Older revision		Revision as of 09:16, 30 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' small subunit of glutamate synthase<br/><br/>~~	+	{{#bank: GltB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''gltB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| glutamate synthase (small subunit)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| glutamate biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18440 gltB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GltB GltB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gltB gltB]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 54.6 kDa, 7.69~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1479 bp, 493 amino acids~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yogA]]'', ''[[gltA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18440 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:gltB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:gltB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18440]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of amino acids]]}}, [[glutamate metabolism]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GltC regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[FsrA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[TnrA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU18440
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~auxotrophic for glutamate~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18440&redirect=T BSU18440]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltAB.html]
−
−	* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12594]
−
−	~~=== Additional information===~~
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' 2 L-glutamate + NADP<sup>+</sup> = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
−
−	* '''Protein family:''' glutamate synthase family.
−
−	* '''Paralogous protein(s):''' none
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−	** nucleotide binding domain (NADP) (299–313)
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[GltA]]-[[GltB]] {{PubMed\|20933603}}
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18440&redirect=T BSU18440]
−
−	* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2VDC 2VDC] (the [[GltA]]-[[GltB]] complex of ''Azospirillum brasiliense'') {{PubMed\|18199747}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34399 O34399]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18440]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.13 1.4.1.13]
−
−	~~=== Additional information===~~
−
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[gltA]]-[[gltB]]'' {{PubMed\|12823818}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 gltB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|2548995}}
−
−	* '''Regulation:''' see ''[[gltA]]''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1128 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP807 (del ''[[gltA]]-[[gltB]]''::''tet'') , available in [[Jörg Stülke]]'s lab
−	** GP517 (ermC), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−	** pGP1119 (in [[pGP380]], for SPINE, expression in ''B. subtilis''), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:''' see ''[[gltA]]''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Linc Sonenshein\|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]~~
−
−	~~[[Stülke\|Jörg Stülke]], University of Göttingen, Germany~~
−	~~[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]~~
−
−	~~[[Fabian_Commichau\|Fabian Commichau]] University of Göttingen, Germany~~
−	~~[http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>16143852 12859215 22625175</pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed>12823818,20933603,11029411,7559360 15150225 2548995 17183217 17608797 17134717 14523131, 12823818 , 18326565 18199747 22389480</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 130: / Line 130: @@
 =Biological materials =
-* '''Mutant:''' GP807 (del ''gltAB''::''tet''), GP517 (ermC), both available in [[Stülke]] lab
+* '''Mutant:'''
 * '''Expression vector:'''
-** pGP1119 (in [[pGP380]], for SPINE, expression in ''B. subtilis''), available in [[Stülke]] lab
+** pGP1119 (in [[pGP380]], for SPINE, expression in ''B. subtilis''), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:''' see ''[[gltA]]''
@@ Line 139: / Line 141: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

← Older revision		Revision as of 09:58, 17 April 2014
Line 126:		Line 126:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1128 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltAB.html]
@@ Line 101: / Line 102: @@
 === Database entries ===
 * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2VDC 2VDC] (the [[GltA]]-[[GltB]] complex of ''Azospirillum brasiliense'') {{PubMed|18199747}}

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GltB GltB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gltB gltB]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 54.6 kDa, 7.69

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18440 gltB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GltB GltB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GltB GltB]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''

@@ Line 33: / Line 33: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gltB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gltB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18440]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yogA]]'', ''[[gltA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18440 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18440 DNA_with_flanks]
 |-
 |-

@@ Line 41: / Line 41: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
@@ Line 123: / Line 119: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 gltB] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|2548995}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2548995}}
 * '''Regulation:''' see ''[[gltA]]''
@@ Line 158: / Line 154: @@
 =References=
 ==Reviews==
- <big>''Gunka K, Commichau FM''  </big>
+<pubmed>16143852 12859215 22625175</pubmed>
 ==Original publications==